Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (6): 654-660.doi: 10.3724/SP.J.1259.2012.00654

Previous Articles     Next Articles

A Regeneration System and Ploidy Identification of Plantlets for Endosperm of Dioscorea zingiberensis

Mingjun Li1,2*, Jing Guo1, Xiang Li1, Jiqiang Li1, Yipeng Wang1, Xiaoli Zhang1,2, Yongkang Liu3   

  1. 1College of Life Sciences, Henan Normal University, Xinxiang 453007, China;

    2Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Xinxiang 453007, China

    3Institute of Agricultural Sciences, Wenxian 454881, China
  • Received:2012-03-23 Revised:2012-04-30 Online:2012-09-04 Published:2012-11-01
  • Contact: Mingjun Li E-mail:limingjun2002@263.net

Abstract: We studied the effect of various plant growth regulators on inducing endosperm callus and plant regeneration using the endosperm of Dioscorea zingiberensis as explants and identified the ploidy of plantlets. The optimal medium for inducing callus, adventitious bud differentiation and root formation was MS+2.0 mg·L–12,4-D+0.5 mg·L–16-BA, MS+2.0 mg·L–16-BA+0.1 mg·L–1NAA and 1/2MS+0.3 mg·L–1NAA, respectively. On observing the growth points of axillary buds in regenerated plants by chromosome slice production techniques, 10%, 16% and 74% of total observed cells had 20, 21–29 and 30 chromosomes, respectively. We obtained triploid plantlets.

代西梅,黄群策(2005).植物多倍体研究进展.河南农业科学(1),9-11.
何芳兰,陶延珍,李毅,苏世平,胡相伟(2007).不同激素对高山杜鹃愈伤组织分化及植株再生的影响.甘肃农业大学学报42,68-72.
黄翠,景丹龙,王玉兵,范深厚,陈发菊(2010).水杉愈伤组织诱导及植株再生.植物学报45(5),604-608.
郭永兵,江道菊,李特林(2007).盾叶薯蓣胚乳的组织培养.北方园艺(10),175-177.
李懋学和张杶方(1991).植物染色体研究技术.东北林业大学出版社.pp.6-48.
李琼,李静,姜玲,胡建斌(2010).盾叶薯蓣愈伤组织的诱导及芽分化与增殖.贵州农业科学38(7),15-17.
李先平,隋启君,赵志坚(2002).通过匍匐茎尖检查马铃薯染色体的方法.中国马铃薯16(6),356-357.
李燕,马俊林,胡菊,杨红琳(2011).黄姜皂素提取的不同预处理方法比较研究.化学工程与装备(08),33-34.
江苏省植物研究所薯蓣课题研究组(1976).中国薯蓣属根茎组植物的分类和染色体数的研究.植物分类学报14(1),65-71.
彭晓英,周朴华,张良波,降到送,刘衍芳(2005).盾叶薯蓣试管株芽的诱导.热带亚热带植物学报13(4),319-323.
秦松云,丁季春,舒抒,余再柏,李泉森(2004).中国盾叶薯蓣资源现状及保护对策.资源开发与市场20(4),263-265.
秦天才,张友德,张君芝(1996).湖北黄姜资源的现状及开发.自然资源(3),58-61

秦新民,梁倩华(1990).金花茶组培苗的核型分析.广西植物10(3),208-210.
任椒矫,顾志敏,刘帅,陈析丰,马伯军(2009).盾叶薯蓣高频再生体系的建立.浙江师范大学学报(自然科学版)32(2),216-221.
尚宏芹(2010).正交设计优化彩色辣椒离体再生条件的研究.生物技术20(4),48-50.
宋智青,梁运章,李玉峰,陈浩(2006).药用植物的多倍性育种.生物学通报41(7),13-14.
唐君海,蓝庆江,陆祖正,符策,周婧(2004).盾叶薯蓣的组织培养研究初报.广西热带农业(3),7-9.
吴清,闫勇,梁国鲁(2002).红杨桃胚乳愈伤组织的诱导和三倍体植株再生.热带作物学报23(2),54-57.
王兴仁,张录达,王华方(2000).正交实验设置重复的必要性和统计分析方法.土壤通报31(3),135-139.
王志安(1983).诱导盾叶薯蓣四倍体的研究初报.中国中药杂志20(6),337-339.
王志成,董静洲,易自力(2004).四倍体盾叶薯蓣快速繁殖的初步研究.三明农业科技(3),19-23.
徐向丽,刘选明,周朴华,易克,朱至清(2000).盾叶薯蓣组织培养微块茎的离体诱导.湖南农业大学学报26(4),282-285.
杨万立(2006).生物多倍性及其在农业上的应用.生物学通报41(3),23-24.
易鼎杰,梁国鲁(2010).甜瓜嫩芽叶尖的染色体技术新方法研究.西南师范大学学报(自然科学版)35(3),67-69.
张琴,闫勇,梁国鲁(2000).西番莲胚乳愈伤组织诱导和三倍体植株再生.西南农业大学学报22(5),398-402.
张耀华(2008).盾叶薯蓣快繁的研究.山西农业科学36(12),58-59.
赵猛,徐增莱,夏冰,王琼,史云云,贾成森(2007).四倍体盾叶薯蓣实生苗的诱导及快速繁殖研究.安徽农业科学35(33),10699-10701.
朱登云,李浚明(1996).被子植物胚乳培养研究的历史与现状.农业生物技术学4(3),205-216.
Chaturvedi R, Razdan MK, Bhojwani SS (2003) An ef?cient protocol for the production of triploid plants from endosperm callus of neem, Azadirachta indica A. Juss. J Plant Physiol 160,557–564.
Chen YQ, Fan JY, Yi F, Luo ZX, Fu YS(2003).Rapid clonal propagation of Dioscorea zingiberensis.Plant Cell Tiss Organ Cult 73,75-80.
Gui Y, Hong S, Ke S, Skirvin RM (1993).Fruit and vegetative characteristics of endosperm-derived kiwifruit (Actinidia chinensis F.) plant. Euphytica 71,57–62.
Liu W, Huang W, Sun WL(2010).Production of diosgenin from yellow ginger (Dioscorea zingiberensis C.H.Wright) saponins by commercial cellulose.World J Microbiol Biotechnol 26,1171–1180.
Miyashita T, Ohashi T, Shibata F, Araki H, Hoshino Y(2009).Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Loniceracaerulea var.emphyllocalyx.Plant Cell Tiss Organ Cult 98,291-301.
Sun DQ, Lu XH, Liang GL, Guo QG, Mo YW, Xie JH(2011).Production of triploid plants of papaya by endosperm culture. Plant Cell Tiss Organ Cult,104,23-29.
Yuan S, Yan YC, Lin HH (2005).Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis.Plant Cell Tiss Organ Cult 80,157-161.
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Sun Hong-tao;Qu Jia-xiang;Fu Wei-dong;Dong Li-hui and Liu Xin. The Screening of Best Medium for Flax Pollen Culture by Using Variance Analysis of Multifactorial Test[J]. Chin Bull Bot, 1988, 5(03): 176 -181 .
[2] YIN Heng LI Shu-Guang BAI Xue-Fang DU Yu-Guang. Research Advances in Plant Metabolomics[J]. Chin Bull Bot, 2005, 22(05): 532 -540 .
[3] NIE Lan-Chun② SUN Jian-She HUANG Rui-Hong. The Biosynthesis and Affecting Factors of Aroma in Some Fruits[J]. Chin Bull Bot, 2004, 21(05): 631 -637 .
[4] Xiuxia He;Yuanhua Yu*;Yong Zhang. Genetic Transformation of PSY Gene in Ginseng[J]. Chin Bull Bot, 2008, 25(05): 579 -584 .
[5] Ye Neng-gan. The Morphology of the Seedlings of Seed Plants[J]. Chin Bull Bot, 1985, 3(04): 7 -12 .
[6] Jiyan Qi Jianghua Yang Chaorong Tang. Sucrose Transporter Genes and Their Functions in Plants[J]. Chin Bull Bot, 2007, 24(04): 532 -543 .
[7] TAN Yun YE Qing-Sheng LIU Wei. Tissue Culture of Dendrobidium huoshanness[J]. Chin Bull Bot, 2005, 22(01): 58 -62 .
[8] Lu Xue-hua and Chen Yang-chun. Tissue Culture and Plantlet Regeneration from Bulb of Lycoris aurea[J]. Chin Bull Bot, 1989, 6(03): 169 .
[9] XU Xiao-Biao ZHANG Qiu-Ming. Researches and Utilizations of Germplasm Resource of Kiwifruit in China[J]. Chin Bull Bot, 2003, 20(06): 648 -655 .
[10] Chen Qi-feng;Cheng Zhang and Wang Ji-ling. Effects of a-Picolinic Acid and The Crude Toxin Extracts From pyricularia oryzae on in Vitro Embryo Culture and Seedling Growth of Rice[J]. Chin Bull Bot, 1993, 10(02): 39 -43 .