Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (1): 28-35.doi: 10.3724/SP.J.1259.2012.00028

Previous Articles     Next Articles

Genetic Analysis of Seed Germinability Under Submergence in Rice

Sunlu Chen1,2†, Junmin Wang3†, Youzhao Pan4, Jianyang Ma2,5, Jianhui Zhang2,5, Hongsheng Zhang1*, Sheng Teng2*   

  1. 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

    2Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;

    3Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;

    4College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China;

    5College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2011-08-26 Revised:2011-11-10 Online:2012-01-16 Published:2012-01-01
  • Contact: Sheng Teng
  • Supported by:

    ;National Natural Science Foundation of China;National Natural Science Foundation of China

Abstract: Submergence tolerance during germination in rice is regulated by a complex molecular network, the molecular mechanism of which is different from that of submergence tolerance during seedling stage. Germinability under submergence (GS) considerably affects seedling establishment of direct-seeded rice. According to the GS of 256 accessions of the rice core collection, both japonica and indica have high genetic variation of GS with no significant difference in GS. We constructed a linkage map of 146 molecular markers from 170 advanced inbred lines from a backcross between R0380 (japonica) and RP2334 (indica) and used composite interval mapping to detect quantitative trait loci (QTL) associated with GS (coleoptile length under hypoxia). We found 4 QTLs on chromosome 2 (2 QTLs), 3 (1 QTL), and 8 (1 QTL). The positive allele of qGS2.2, which explained 17.34% of the phenotypic variation, was from RP2334, and alleles for the other 3 QTLs came from R0380, which explained 12.86%, 9.37% and 14.60%, respectively, of the phenotypic variation.

CLC Number: 

  • Q943.2

侯名语 (2003). 水稻低温、低氧发芽力的QTL定位. 硕士论文. 南京: 南京农业大学. pp. 24-32.
侯名语, 江玲, 王春明, 万建民 (2004). 水稻种子低氧发芽力的QTL定位和上位性分析. 中国水稻科学 18, 483-488.
沈利爽, 郑先武, 朱立煌 (2000). Mapplotter——一个输出遗传图谱、图示基因型和QTL曲线图形的软件. 遗传 22, 172-174.
王洋, 郭媛, 洪德林 (2010). 水稻幼苗耐缺氧能力的QTL分析. 中国水稻科学 24, 18-24.
王洋, 王盈盈, 洪德林 (2009). 太湖流域水稻种子活力和耐缺氧能力遗传变异研究. 南京农业大学学报 32, 1-7.
Alpi A, Beevers H (1983). Effects of O2 concentration on rice seedlings. Plant Physiology 71, 30-34.
Angaji SA (2008). Mapping QTLs for submergence tolerance during germination in rice. African Journal of Biotechnology 7, 2551-2558.
Angaji SA, Septiningsih EM, Mackill DJ, Ismail AM (2010). QTLs associated with tolerance of flooding during germination in rice (Oryza sativa L.). Euphytica 172, 159-168.
Gibbs J, Morrell S, Valdez A, Setter TL, Greenway T (2000). Regulation of alcoholic fermentation in coleoptiles of two rice cultivars differing in tolerance to anoxia. Journal of Experimental Botany 51, 785-796.
Hattori Y, Nagai K, Furukawa S, Song XJ, Kawano R, Sakakibara H, Wu J, Matsumoto T, Yoshimura A, Kitano H, Matsuoka M, Mori H, Ashikari M (2009). The ethylene response factors SNORKEL1, SNORKEL2 allow rice to adapt to deep water. Nature 460, 1026-1030.
Jiang L, Liu S, Hou M, Tang J, Chen L, Zhai H, Wan J (2006). Analysis of QTLs for seed low temperature germinability, anoxia germinability in rice (Oryza sativa L.). Field Crops Research 98, 68-75.
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987). MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental, natural populations. Genomics 1, 174-181.
Lasanthi-Kudahettige R, Magneschi L, Loreti E, Gonzali S, Licausi F, Novi G, Beretta O, Vitulli F, Alpi A, Perata P (2007). Transcript profiling of the anoxic rice coleoptile. Plant Physiology 144, 218-231.
Lee KW, Chen PW, Lu CA, Chen S, Ho THD, Yu SM (2009). Coordinated responses to oxygen, sugar deficiency allow rice seedlings to tolerate flooding. Science Signaling 2, ra61.
Lincoln S, Daly M, Lander E (1992). Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report. 3rd edition, Whitehead Institute, Cambridge, Mass.
Magneschi L, Kudahettige RL, Alpi A, Perata P (2009). Comparative analysis of anoxic coleoptile elongation in rice varieties: relationship between coleoptile length, carbohydrate levels, fermentative metabolism, anaerobic gene expression. Plant Biology 11, 561-573.
Magneschi L, Perata P (2009). Rice germination, seedling growth in the absence of oxygen. Annals of botany 103, 181-196.
McCouch S, CGSNL (2008). Gene nomenclature system for rice. Rice 1, 72-84.
Perata P, Alpi A (1993). Plant responses to anaerobiosis. Plant Science 93, 1-17.
Setter TL, Ella ES, Valdez AP (1994). Relationship between coleoptile elongation, alcoholic fermentation in rice exposed to anoxia. II. cultivar differences. Annals of botany 74, 273-279.
Wang S, Basten C, Zeng Z (2011). Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC.
Xu K, Xu X, Fukao T, Canlas P, Maghirang-Rodriguez R, Heuer S, Ismail AM, Bailey-Serres J, Ronald PC, Mackill DJ (2006). Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature 442, 705-708.
Full text



[1] Xiling Dai;Jianguo Cao;Quanxi Wang* . Formation and Development of Sporoderm of Ceratopteris thalictroides (L.) Brongn. (Parkeriaceae)[J]. Chin Bull Bot, 2008, 25(01): 72 -79 .
[2] Liu De-li. Heat-Shock Proteins of Plants and their Functions[J]. Chin Bull Bot, 1996, 13(01): 14 -19 .
[3] Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding. Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics[J]. Chin Bull Bot, 2011, 46(1): 67 -73 .
[4] Xing Xue-rong Lu Chun-sheng Guo Da-li. Effect of Oraganic Acid to Nitrate Reductase and Nitrite Reductase Activity in the Vegetables[J]. Chin Bull Bot, 1995, 12(专辑2): 156 -162 .
[5] SONG Ke-Min. Phosphorus Nutrition of Plants: Phosphate Transport Systems and their Regulation[J]. Chin Bull Bot, 1999, 16(03): 251 -256 .
[6] CHEN Fa-Ju;YANG Ying-Gen;ZHAO De-Xiu;GUI Yao-Lin and GUO Zhong-Chen. Advances in Studies of Species Habitats Distribution and Chemical Composition of Snow Lotuses(Saussurea) in China[J]. Chin Bull Bot, 1999, 16(05): 561 -566 .
[7] YANG Hong-QiangJIE Yu-lingLI Jun. The Stresses Messenger from Roots and Its Production and Transport in Plant[J]. Chin Bull Bot, 2002, 19(01): 56 -62 .
[8] Xianwei Zhang;Li Yang;Tao Zhang;Kaifeng Jiang;Guixue Wang;Jiakui Zheng;*;Xianlin Ni;Cui Tian;Yingjiang Cao. QTL Mapping for Zinc Content in Rice Grains[J]. Chin Bull Bot, 2009, 44(05): 594 -600 .
[9] Hui Li, Guangcan Zhang, Huicheng Xie, Jingwei Xu, Chuanrong Li, Juwen Sun. The Effect of Phenol Concentration on Photosynthetic Physiological Parameters of Salix babylonica[J]. Chin Bull Bot, 2016, 51(1): 31 -39 .
[10] . [J]. Chin Bull Bot, 1996, 13(专辑): 97 -98 .