Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (6): 625-631.DOI: 10.3724/SP.J.1259.2011.00625

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Phenotypic Analysis and Gene Mapping of a Short Root Hair Mutant Ossrh2 in Rice (Oryza sativa)

Wona Ding1*, Yanli Tong2, Yongqiang Ning1, Shihua Zhu1   

  1. 1Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China;

    2State Key Laboratory of Plant Physiologyand Biochemistry, Zhejiang University, Hangzhou 310058, China
  • Received:2011-05-04 Revised:2011-06-30 Online:2011-11-01 Published:2011-11-18
  • Contact: Wona Ding

Abstract: A rice (Oryza sativa) mutant with short root hairs (Ossrh2) was isolated from a T-DNA insertion mutant library of rice in the Zhonghua11 background. Except for root hairs, Ossrh2 did not differ from the wild type. The number and length of root hairs of Ossrh2 were only 61.4% and 22.8%, respectively, that of the wild type, and hairs were thicker. The root hairs of Ossrh2 were also morphologically abnormal and showed distortion, swelling and branching. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. PCR analysis of T2 and F2 mutants showed that the phenotype was not caused by T-DNA insertion. To map the OsSRH2 gene, we generated an F2 population by crossing the mutant Ossrh2 with the Kasalath wild type. OsSRH2 was first mapped between the microsatellite markers RM6370 and RM474 on the short arm of chromosome 10 with genetic distance of 1.1 and 3.0 cM, respectively. Then, 3 new polymorphic sequence-tagged site markers were developed in the region. OsSRH2 was finally mapped between markers S1227 and S1531 with a physical distance of 304 kb, which would help in its cloning.