Abstract: Poly ADP-ribose polymerase (PARP) is induced specifically by genotoxin. We cloned the 2 179 bp promoter sequence of Arabidopsis AtPARP1 gene into a pAKK687 vector to drive the expression of a β-glucuronidase (GUS) reporter gene for Arabidopsis transformation. GUS staining revealed the GUS reporter gene expressed only in the basal root of 3 to 5-day-old seedlings and in the anther at early flowering stage. An amount of 1.5 μg·mL^–1 bleomycin combined with 22 μg·mL^–1 mitomycin could strongly induce the expression of the GUS reporter gene, especially in young seedlings and young siliques of Arabidopsis. An amount of 1 μg·mL^–1 bleomycin was enough to induce the promoter and had no adverse effects on Arabidopsis seedling growth. The AtPARP1 promoter may be a new antibiotic-inducible promoter with application potential.