Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (2): 147-154.doi: 10.3724/SP.J.1259.2011.00147

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Function of GLP13 in Response to Plant Oxidative Stress in Arabidopsis

Yuanjiang Tang1, Lingli Min1, Guilan Gao2, Jinju Du2, Lang Yang2, Chengwei Yang2*   

  1. 1Department of Biological Engineering and Biotechnology, Huaqiao University, Xiamen 361021, China;

    2College of LifeScience, South China Normal University, Guangzhou 510631, China
  • Received:2010-09-15 Revised:2010-11-05 Online:2011-05-09 Published:2011-03-01
  • Contact: Chengwei Yang

Abstract: Germin-like proteins (GLPs) comprise a large family of soluble extracellular matrix glycoprotein, which is similar to wheat germin and plays an important role in growth and development and in biotic and abiotic stress responses of plants. To understand the role of GLP13 in planta, we investigated its expression patterns; isolated and characterized a knock-down mutation in the GLP13 gene (named glp13); and constructed overexpression GLP13 plants (named 35S::GLP13). Compared to the wild type, the glp13 mutant showed a lower cotyledon green ratio and more restrained root growth when 3 different types of plants were treated with methyl viologen (MV); however, the cotyledon green ratio was higher and the root growth less restrained in 35S::GLP13 plants. The chlorophyll fluorescence parameter Fv/Fm of 35S::GLP13 plants decreased slower than in the wild type after 2-week MV treatment of seedlings. The expression of oxidative stress response genes (CSD1, FSD1, UGT71C1 and CAT1) were analyzed by semi-quantitative RT-PCR; the expression of FSD1 was increased in 35S::GLP13 plants, with no differences in the expression level of CAT1, CSD1 and UGT71C1 among 3 different types of plants after 4-hr treatment with MV. Our results indicate that GLP13 plays an important role in response to oxidative stress in Arabidopsis.

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