A Non-invasive Method for Measuring and Analyzing Circadian Phenotype in Living Plants

doi:10.11983/CBB19238

Abstract

Abstract: The plant circadian clock is a time-keeping molecular system, with a cycle of ~ 24 h. It was evolved to adapt to the diel rhythmic environmental cues generated by the self-rotation of the Earth. In addition to the time-keeping function, the circadian clock also regulates a plethora of plant growth and development processes by synchronizing the endogenous energy and metabolomic status. By sensing and integrating the dynamics of external environmental cues, circadian clock can coordinate gene expression at multiple levels, thus to increase the fitness of plants. Recently, there is an increasingly demand for measuring and assessing circadian phenotype for many non-circadian research field. Here, we summarized the currently available methods for detecting circadian rhythm, and show one most commonly used standard procedure for evaluating circadian phenotype in plants, which may help to provide the applicable technical assistance for the study of the circadian clock.

Key words: circadian clock, phenotype analysis, FFT-NLLS, BRASS

Yingjun Yu,Hang Xu,Lei Wang. A Non-invasive Method for Measuring and Analyzing Circadian Phenotype in Living Plants[J]. Chinese Bulletin of Botany, 2020, 55(2): 177-181.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB19238

https://www.chinbullbotany.com/EN/Y2020/V55/I2/177

Figures/Tables 1

Figure 1 Circadian phenotype analysis of Col-0 wild type Arabidopsis thaliana CCA1pro:LUC under continuous red light (A) Trace plot of bioluminescence in Col-0 wild type Arabidopsis thaliana with CCA1pro:LUC reporter under continuous red light conditions, from LL24 to LL144 (the abbreviation of c.p.s represents the counts of photons per seedling). (B) Scatter plot of circadian period (x-axis) and relative amplitude error (RAE) (y-axis) of Col-0; According to the results, the periods are more concentrated and relatively consistent, and the relative amplitude errors are all lower than 0.5, which indicates that the result is reliable (generally, when the RAE is greater than 0.5, the circadian oscillation intensity is relatively low, which is not suitable for calculating the period). (C) Estimated circadian period of CCA1pro:LUC in Col-0; According to the calculation, circadian period length of Col-0 is 23.22 hours under continuous red light. Calculation was performed using FFT-NLLS method. Data are presented as means±SE (n=21).

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