Chin Bull Bot ›› 2020, Vol. 55 ›› Issue (1): 90-95.doi: 10.11983/CBB19168

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Optimization of Cell Suspension Culture Conditions of Achyranthes bidentata

Li Ping1,2,Dong Yahui1,Li Chenglong1,He Yulong1,Li Mingjun1,3,*()   

  1. 1College of Life Science, Henan Normal University, Xinxiang 453007, China
    2Zhengzhou Shuqing Medical College, Zhengzhou 450064, China
    3Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province/Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang 453007, China
  • Received:2019-08-27 Accepted:2019-11-13 Online:2019-12-20 Published:2020-01-01
  • Contact: Li Mingjun E-mail:limingjun2002@263.net

Abstract:

To optimize the suspension culture conditions of Achyranthes bidentata cells, effect of different factors like inoculum concentrations, subculture cycles, pH, light and Cu 2+ on cell growth and polysaccharides contents was studied. The results showed that, cells grew well with a good ability for polysaccharides synthesis under 50 g·L -1 inoculum concentration, 14 d subculture cycle, pH5-6 and light. In the presence of 50 μmol·L -1 Cu 2+, the cell dry weight could be the maximum with 44.63 g·L -1, and polysaccharides contents reached the peak at 4.02 mg·g -1.

Key words: Achyranthes bidentata, cell suspension culture, cell growth, polysaccharides

Figure 1

Effect of different factors on the cell suspension culture of Achyranthes bidentata (A) Effect of inoculum concentrations on the cell growth; (B) Effect of inoculum concentrations on the contents of ABPS; (C) Effect of subculture cycle on the cell growth; (D) Effect of subculture cycle on the contents of ABPS; (E) Effect of pH and light on the cell growth; (F) Effect of pH and light on contents of ABPS; (G) Effect of Cu2+ concentration on the cell growth; (H) Effect of Cu2+ concentration on the contents of ABPS. The different letters indicate significant differences at the 0.05 level. ABPS: Achyranthes bidentata polysaccharides"

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