Effects of the Respiratory Electron Transport Pathways in Relieving Photoinhibition of Chloroplast PSII in Tobacco Leaves

doi:10.11983/CBB19117

Abstract

Abstract: Previous studies have shown that the mitochondrial alternative oxidase (AOX) respiratory pathway contributes to the photoprotection of photosystem II (PSII) under high light. However, the role of another respiratory pathway in mitochondria, the cytochrome-respiratory (COX) pathway, to photoprotection under high light remains unknown. Here, we studied the contributions of COX and AOX pathways to PSII photoprotection, as well as the relationship between COX and AOX pathways in PSII photoprotection in tobacco leaves under high light using chlorophyll fluorescence transients and chlorophyll fluorescence quenching approaches. After high light treatment, the PSII activity decreased in all leaves. The inhibition of AOX pathway significantly accelerated the decrease of PSII activity in leaves. However, there was no significant difference between the PSII activity in the leaves pretreated with COX pathway inhibitor and water-pretreated control leaves. When both AOX and COX pathways are inhibited in leaves, the decrease in PSII activity was severe, than that of leaves pretreated by only AOX pathway inhibitor. In addition, inhibition of respiratory electron transport resulted in an increase of non-photochemical quenching (NPQ). The up-regulation of NPQ caused by AOX pathway inhibitor was more obviously than that caused by COX pathway inhibitor, and the increase of NPQ was highest when both AOX and COX pathways were inhibited. These results indicate that both COX and AOX pathways contribute to the PSII photoprotection in tobacco leaves under high light. In addition, when COX pathway is inhibited, its photoprotective function can be compensated by AOX pathway and NPQ, however, photoprotection of AOX pathway cannot be completely compensated by COX pathway and NPQ.

Key words: alternative oxidation respiratory pathway, cytochrome respiratory pathway, non-photochemical quenching, photoprotection, PSII photoinhibition

Jiao Luo,Yuting Li,Zishan Zhang,Xingkai Che,Ying Liang,Yuenan Li,Ying Li,Shijie Zhao,Huiyuan Gao. Effects of the Respiratory Electron Transport Pathways in Relieving Photoinhibition of Chloroplast PSII in Tobacco Leaves[J]. Chinese Bulletin of Botany, 2020, 55(1): 31-37.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB19117

https://www.chinbullbotany.com/EN/Y2020/V55/I1/31

Figures/Tables 3

Figure 1 The effect of water (CK) or 2 μmol∙L-1 antimycin A (AA) treatment on the P700+ relaxation curve after far-red light in tobacco leaves All data are the average of 8 replicates.

Figure 2 The effect of 0 (A) or 1 h (B) high light treatment on the OJIP transients in different pretreated tobacco leaves and the effect of high light treatment on the maximum quantum yield of photosystem II (Fv/Fm) (C) in different pretreated tobacco leaves All data are the average of 8 replicates. Different lowercase letters indicate significant differences (P<0.05).

Figure 3 The effect of high light treatment on the actual PSII photochemical efficiencies (ФPSII ) (A), the efficiency of excitation energy capture by open PSII reaction centres (Fv′/Fm′) (B), the photochemical quenching coefficients (qP) (C) and the non-photochemical quenching (NPQ) (D) in different pretreated tobacco leaves All data are the average of 8 replicates. Different lowercase letters indicate significant differences (P<0.05).

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