Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 699-710.DOI: 10.11983/CBB19055

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Genome-wide Identification and Phylogenetic Analysis of Zinc Finger Homeodomain Family Genes in Brassica napus

Min Song(),Yao Zhang,Liying Wang,Xiangyong Peng   

  1. School of Life Sciences, Qufu Normal University, Qufu 273165, China
  • Received:2019-03-25 Accepted:2019-07-26 Online:2019-11-01 Published:2020-07-09
  • Contact: Min Song

Abstract: Zinc finger-homeodomain (ZF-HD) genes encode plant-specific transcription factors that participate in the regulation of plant growth, development and abiotic stress response. In this study, a total of 62 ZF-HD genes were identified in Brassica napus. Among them, 83.9% lack introns, 93.5% of deduced proteins have the isoelectric point greater than 7. Most of the BnZF-HD proteins with nuclear localization signal were predicted to be more than 100 amino acid residues. Based on phylogenetic analysis, this gene family can be divided into six subgroups. In each subgroup, the number of ZF-HD genes in B. oleracea and B. rapa was the same or nearly so, while the number of ZF-HD genes in B. napus was close to or equal to the sum of ZF-HD genes in B. oleracea and B. rapa. The number and type of motifs in each subgroup were highly conserved. Collinear analysis indicated that the whole genome triplication allowed the ZF-HD gene family to expand in diploid ancestors, and allopolyploidization made the ZF-HD gene family to reexpand in B. napus. Ka/Ks analysis showed that the ZF-HD gene family has experienced purification selection in the evolution process. All BnZF-HD genes have light response elements, and two-thirds of them have MeJA, ABA and anaerobic inducible cis-acting elements. It is speculated that they may participate in related biological processes. This study laid a foundation for further exploring the molecular mechanism of this family in regulating plant growth and stress responses, and provided more reference for future research on the evolution of plant polyploidization.

Key words: Brassica napus, ZF-HD, evolution, collinear analysis, cis-acting elements