Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 786-796.doi: 10.11983/CBB19045

• SPECIAL TOPICS • Previous Articles     Next Articles

Advances in Studies on the COPT Proteins in Arabidopsis thaliana

Wang Hui1,Li Jinjin2,Xu Jinyu1,Liu Peng1,Zhang Haiyan1,*   

  1. 1 Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China
    2 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2019-03-13 Accepted:2019-06-18 Online:2020-07-09 Published:2019-11-01
  • Contact: Zhang Haiyan


Copper (Cu) is an essential trace element in plants and is involved in many physiological and biochemical reactions as a cofactor of various enzymes. Cu deficiency and excess can affect the normal growth and development, so plants have developed sophisticated regulatory networks to strictly control Cu content. The copper transporter COPT, having high affinity with Cu can regulate the absorption and transport of Cu, and plays an important role in maintaining Cu homeostasis. COPT proteins are involved in different processes of Cu transport, such as uptake of Cu from the external environment, export of Cu from organelles, transport of Cu over long distances, and mobilization and redistribution of Cu between different organs. In addition, COPT proteins play an important role in maintaining homeostasis of other ions, regulating the circadian clock, involving in synthesis of plant hormones and perception of hormone signals. Here we summarize the recent advances in the expression and localization, regulatory mechanisms, and biological functions of COPT proteins in the model plant Arabidopsis thaliana.

Key words: copper transporter, COPT, Cu homeostasis, Cu absorption, redistribution

Table 1

Expression patterns of COPT family genes detected by use of promoter-GUS in Arabidopsis thaliana"

基因名称 表达器官 参考文献
花粉 花丝 雌蕊 胚珠 子叶 表皮毛 保卫细胞 维管组织 维管组织 胚轴 主根 侧根 根毛
COPT1 ++ - / / + + + - - + + + / Kampfenkel et al.,1995; Sancenon et al., 2004
COPT2 ++ - - / + + / / - / + + + Sancenón et al., 2003; Perea-García et al., 2013
COPT3 ++ + / / / / / + / / / / / Andrés-Colás et al., 2018
COPT5 - + ++ ++ + + / ++ + + ++ + ++ Garcia-Molina et al., 2011
COPT6 + + + + + + + ++ ++ / - + / Jung et al., 2012; Garcia-Molina et al., 2013

Figure 1

Subcellular localization of COPT and copper traffic in Arabidopsis (modified from Rodríguez et al., 1999; Balandin and Castresana, 2002; Wintz et al., 2003; Yruela, 2009; Garcia et al., 2014) Cu2+can be uptaken by ZIP proteins or reduced to Cu+ by FRO and enters into the cytosol through the COPT1, COPT2 and COPT6 transporters. In the cell, COPT5 localizes in the tonoplast and vacuolar precursor membranes and transports Cu+ to the cytosol. It is speculated that COPT3 localizes to the ER membrane and transports Cu+ to the cytosol. HMA6 and HMA1 are localized in the membranes of the chloroplast and responsible for transporting Cu+ and Cu2+ to the cytosol, respectively. HMA8 is located in the thylakoid membrane and transports the Cu+ of the stroma into the thylakoid cavity. HMA7 transports Cu+ through the golgi membrane and participating in the ethylene reaction. HMA5 localizes in the cell membrane and mediates the efflux of Cu+."

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