Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (3): 335-342.doi: 10.11983/CBB18216

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Analysis of Curcuminoids and Mineral Elements in Curcuma from Different Habitats

Yang Xiaofang1,Chen Yucai2,Liu Jianfu1,*(),Li Dandan1,Hu Xian1   

  1. 1. Department of Horticulture, Huaqiao University, Xiamen 361021, China
    2. Agricultural Service Center of Fengzhou Town Nan’ an City, Quanzhou 362330, China;
  • Received:2018-10-17 Accepted:2019-02-20 Online:2019-11-24 Published:2019-05-01
  • Contact: Liu Jianfu E-mail:jianfu@hqu.edu.cn

Abstract:

In this study, the mature rhizomes of 12 germplasms of Curcuma longa and C. zedoaria from different origins cultivated in Fujian, Quanzhou, were used as experimental materials. The orthogonal design method was used to optimize the extraction of curcumin (CCM), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), and the content of 3 curcuminoids and 16 mineral elements in different germplasms were compared. The extraction rate peaked with the following extraction process: 100% methanol as solvent, 1:5 (g·mL-1) solid-liquid ratio, and ultrasonic extraction for 2 min. The maximum absorption wavelengths for CCM, DMC and BDMC were 424, 418 and 414 nm, respectively. The linear equations for the 3 curcuminoids were Y=0.170 3X+0.024 3 (CCM), Y=0.173 8X+0.041 (DMC), and Y=0.140 6X+0.051 4 (BDMC). The content of CCM, DMC and BDMC in GY03 was 1.23%, 1.22% and 1.50%, respectively, and that of total curcumin was the highest, 3.95%; The content of total curcumin in GY01 was next, up to 3.82%. GY03 and GY01 can be promoted in cultivated land as excellent breeding material. Among the 16 mineral elements detected in 12 turmerics, the contents of Mg, Mn, Al, Fe, and Zn were relatively abundant.

Key words: Curcuma longa, C. zedoaria, curcuminoids, mineral elements, ICP-MS

Table 1

Digestion procedure of microwave"

StepTemperature (°C)Power (J·S-1)Time (min)Fan levelStirrer
Power ramp50081Off
Power hold50051Off
Power ramp100081Off
Power hold1000251Off
Cooling70--3Off

Table 2

Operating parameters of ICP-MS"

ParametersValue
Plasma gas (L·min-1)15.00
Auxiliary gas (L·min-1)1.000
Atomizing gas (L·min-1)1.000
Compensation/dilution gas (L·min-1)1.000
Spray chamber temperature (°C)2
Peristaltic pump speed (rps)0.1

Figure 1

UV spectrum of CCM(A), DMC (B), and BDMC (C) standard solutions CCM: Curcumin; DMC: Demethoxycurcumin; BDMC: Bisdeme-thoxycurcumin"

Table 3

Orthogonal test results"

Experiment numberABCDCCM content (%)DMC content (%)BDMC content (%)
Methanol concentration (%)Solid-liquid ratio (g·mL-1)Ultrasonic time (min)Empty column
1100 (1)1:4 (1)2 (1)10.390.380.46
211:5 (2)4 (2)20.520.510.61
311:6 (3)6 (3)30.390.370.44
485 (2)1230.250.240.28
522310.420.400.48
623120.310.290.35
770 (3)1320.230.220.26
832130.230.220.26
933210.230.210.25
CCMK11.300.870.931.04
K20.981.171.001.06
K30.690.931.040.87
k10.430.290.310.35
k20.330.390.330.35
k30.230.310.350.29
R0.200.100.040.06
DMCK11.260.840.890.99
K20.931.130.961.02
K30.650.870.990.83
k10.420.280.300.33
k20.310.380.320.34
k30.220.290.330.28
R0.200.100.030.06
BDMCK11.511.001.071.19
K21.111.351.141.22
K30.771.041.180.98
k10.500.330.360.40
k20.370.450.380.41
k30.260.350.390.33
R0.240.120.030.08

Table 4

Comparison of curcuminoid compounds in rhizomes of 12 germplasms of Curcuma longa and C. zedoaria"

VarietyCCM content
(%)
DMC content
(%)
BDMC content
(%)
Total curcumin content (%)
GY031.231.221.503.95
GY011.191.181.453.82
YJHS1.061.051.293.40
GJHP0.580.560.671.81
GJYX0.350.330.401.08
GJQS0.250.250.280.78
HJCG0.230.220.260.71
GY040.220.210.250.68
GJHX0.160.160.190.51
YJLX0.070.060.070.20
HJNN0.070.060.070.20
GY020.030.020.020.07

Table 5

Linear regression equations and correlation coefficients"

ElementLinear regression
equation
Correlation
coefficient
27AlY=0.0062X+0.06350.9983
137BaY=0.0043X+0.00330.9997
9BeY=0.0260X1.0000
111CdY=0.0285X-2.9371E-0051.0000
59CoY=1.6282X+0.02061.0000
63CuY=1.4942X+0.19121.0000
56FeY=0.6704X+4.77930.9979
69GaY=0.1868X+0.03201.0000
24MgY=0.0325X-0.06310.9958
55MnY=0.2312X+0.03691.0000
60NiY=0.5521X+0.04921.0000
88SrY=0.0339X-0.00891.0000
47TiY=0.0096X+0.00571.0000
205TlY=0.0789X-9.5059E-0061.0000
51VY=0.5372X+0.00781.0000
66ZnY=0.1656X+0.71150.9904

Table 6

Comparison of mineral elements in rhizomes of 12 germplasms of Curcuma longa and C. zedoaria"

VarietyQuality score (mg·kg-1)
AlBaBeCdCoCuFeGaMgMnNiSrTiTlVZn
GY0353.2815.470.000.050.194.6249.434.022516.60330.740.1011.641.600.150.0540.12
GY01107.3323.950.010.050.227.8174.106.452342.13137.410.1612.441.910.160.0670.06
YJHS29.9621.050.010.240.246.6723.122.891482.64284.590.209.351.500.150.0562.79
GJHP113.1115.370.010.410.474.3251.434.191689.62304.130.1614.734.240.150.1851.33
GJYX13.6218.120.010.430.377.5560.712.591745.52250.380.0813.250.880.260.0558.44
GJQS224.2118.370.010.080.236.46138.653.682443.95195.780.1016.484.150.100.2854.51
HJCG89.3312.870.000.500.306.2091.733.142588.57293.920.1510.801.280.150.1138.77
GY04152.6717.310.010.300.466.24125.424.263027.62492.750.0817.282.100.170.1383.69
GJHX56.5411.050.010.310.345.49101.392.802721.11437.410.0514.180.710.160.0370.53
YJLX35.708.170.000.090.114.4034.422.011598.89291.260.038.300.440.200.0346.33
HJNN37.889.020.010.090.243.3144.302.251584.92417.020.0210.440.450.360.0358.61
GY0257.458.900.010.250.385.6777.382.121991.98382.800.0411.440.620.060.0456.35
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