Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (5): 634-641.DOI: 10.11983/CBB18193

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus

Ying Feng1,*(),Lianwen Qian1,Qingliang Lin2   

  1. 1. School of Resource and Environmental Science, Quanzhou Normal University, Quanzhou 362000, China
    2. Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2018-09-11 Accepted:2019-02-19 Online:2019-09-01 Published:2020-03-10
  • Contact: Ying Feng

Abstract: To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus, leaf browning was controlled on medium with different kinds and content of hormones (6-BA, GA3, NAA) by a single-factor completely random test. Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double- and single-factor completely random test, respectively. The rate of leaf browning reached 0, and the rate of callus induction was up to 100% on the improved MS medium with 1.0 mg∙L -1 6-BA and 1.0 mg∙L -1 GA3 and 0.3 mg∙L -1 NAA. The rate of callus browning reached 0, and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg∙L -1 6-BA and 0.2 mg∙L -1NAA. The callus was yellow with green or yellow, granular, small and compact, hard and dry. This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C. paliurus.

Key words: browning, Cyclocarya paliurus, callus, induction, propagation