In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. ‘Variegata’

doi:10.11983/CBB16170

Abstract

Abstract: We performed in vitro plantlet regeneration and multiplication of Haworthia retusa × cooperi cv. ‘Variegata’ for industrial production by taking the young alabastrum as explants. Calli were induced from explants on MS+5.0 mg∙L^-1 6-BA+0.5 mg∙L^-1 IBA medium. MS medium without 6-BA was found appropriate for the differentiation of calli. The regenerated shoots had the highest multiplication ratio and induction rate of chimeric bud (16.7 and 79.9%, respectively) when propagated on MS+0.2 mg∙L^-1 6-BA. Our results show that the plantlet regeneration of H. retusa × cooperi cv. ‘Variegata’, through adventitious bud induction from callus was feasible and an appropriate concentration of cytokinin could improve the induction rate of chimeric buds. This study offers insight into the germplasm protection and industrial production of rare chimeric succulents.

Yan Wang, Haojie Mou, Yongping Lü, Haiying Li, Yiting Wang, Jianping Chen. In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. ‘Variegata’[J]. Chinese Bulletin of Botany, 2017, 52(3): 331-336.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB16170

https://www.chinbullbotany.com/EN/Y2017/V52/I3/331

Figures/Tables 5

Figure 1 The callus induction, differentiation and plantlet regeneration of Haworthia retusa × cooperi cv. ‘Variegata’(A) Callus induction; (B) Callus differentiation; (C), (D) The induction of adventitious buds; (E) Seedling and rooting; (F) Transplantation. Bar=1 mm

Table 1 Effects of 6-BA on the callus differentiation of Haworthia retusa × cooperi cv. ‘Variegata’

Concentration of 6-BA (mg∙L^-1)	No. of calli	No. of differentiated calli	Differentiation rate (%)	No. of adventitious bud	Induction rate of buds
0	20	20	100.0	197	9.85±3.51 a
0.1	20	17	85.0	133	6.65±2.47 b
0.2	20	13	65.0	81	4.05±1.83 c
0.5	20	7	35.0	36	1.80±0.94 d

Table 2 Effects of 6-BA on stability of Haworthia retusa × cooperi cv. ‘Variegata’ during multiplication

Concentration of 6-BA (mg∙L^-1)	No. of buds for multiplication	No. of green buds	No. of yellow buds	No. of chimeric buds	Total No. of buds	Multiplication ratio	Induction rate of chimeric bud (%)
0.05	20	7	44	26	77	3.85±0.74 c	33.7±5.63 c
0.10	20	29	90	83	202	9.55±2.28 b	41.4±7.96 b
0.20	20	28	39	267	334	16.70±4.69 a	79.9±8.98 a

Figure 2 Effects of 6-BA concentrations on the induction rates of different adventitious shoots in Haworthia retusa × cooperi cv. ‘Variegata’

Figure 3 The adventitious buds of Haworthia retusa × cooperi cv. ‘Variegata’ under different multiplication condition(A) 0.05 mg∙L-1 6-BA; (B) 0.1 mg∙L-1 6-BA; (C) 0.2 mg∙L-1 6-BA

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