Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (5): 684-690.doi: 10.11983/CBB16008

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A Rapid Propagation System for Scirpus × mariqueter

Qun Zhang1,2†, Xiuli Lü1†, Xiaoli He1, Yi Zhu1, Xinhong Cui1*   

  1. 1Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
    2Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Sciences, Fudan University, Shanghai 200438
  • Received:2016-01-13 Accepted:2016-05-12 Online:2016-09-27 Published:2016-09-01
  • Contact: Zhang Qun,Lü Xiuli,Cui Xinhong E-mail:kysxinhongcui@163.com
  • About author:

    # Co-first authors

Abstract:

The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.

Table 1

Germination condition of Scirpus × mariqueter seeds on M1 medium under different treatments"

Treatment No. of contamination No. of aseptic seedlings Percentage of germination (%)
Intact seed 1.00±1.00 5.33 5.33b
Piercing naked seed 26.00±2.65 15.00 15.00a
Naked seed 38.00±3.00 4.00 4.00c

Figure 1

Different stage of plantlet regeneration from Scirpus × mariqueter seeds (A) Seed embryo partially without seed coat (left), seed embryo entirely without seed coat (right); (B) Germ-free germination seedlings; (C) Multiple shoots; (D) Multiple shoots were dwarf and cluster; (E) Dead multiple shoots under low temperature; (F) Leaves were burned by high temperature; (G) Root induction; (H) Seedlings grow in plastic container; (I) Transplanting"

Table 2

Effects of plant growth regulators on multiplication of Scirpus × mariqueter"

No. of medium Phytohormone composition (mg·L-1) Multiplication
coefficient
Differentiation status and color change of leaves
6-BA TDZ IBA
M2 0.1 0 0.1 1.00d No differentiation, yellow, weak and thin
M3 0.5 0 0.1 1.00d No differentiation, yellow, weak and thin
M4 1.0 0 0.1 1.17d Few differentiation, yellow-green, weak and thin
M5 2.0 0 0.2 1.13d Few differentiation, weak and thin and died
M6 1.0 0.002 0.1 1.42c Green, weak and thin, multiple shoots were induced
M7 1.0 0.002 0.2 1.50c Green, weak and thin, multiple shoots were induced
M8 2.0 0.002 0.2 2.68a Green, weak and thin, multiple shoots were induced
M9 2.0 0.002 0.3 2.53a Green, weak and thin, multiple shoots were induced
M10 3.0 0.002 0.3 2.02b Green, weak and thin, multiple shoots were induced

Table 4

Effects of plant growth regulators on culturing strong plants of Scirpus × mariqueter"

No. of
medium
Phytohormone composition (mg·L-1) Multiplication
coefficient
Height
(cm)
Differentiation status and color change of leaves
6-BA IBA
M11 0 0 1.12d 3-4 Few yellow-green, some rooting shoots
M12 0.05 0.01 1.42cd 3-4 Thriving, multiple shoots uniform
M13 0.1 0.01 1.67bc 2-3 Relatively uniform, few small buds
M14 0.2 0.02 1.83b 2-3 Multiple shoots short, some small buds
M15 0.5 0.05 3.02a 1-2 Multiple shoots short, more small buds

Table 5

Effects of different plant growth regulators on rooting of Scirpus × mariqueter"

No. of
medium
Phytohormone composition (mg·L-1) Percentage of
rooting shoots (%)
Description of root growing and differentiation status
IBA NAA
M16 0.05 0 83.33ab Roots relatively uniform and strongly
M17 0.1 0 96.67a Roots relatively uniform and strongly
M18 0.2 0 100.00a Roots uniform and strongly
M19 0.5 0 100.00a Roots uniform and too much
M20 0 0.2 66.67b Roots not consistent, long and thin, callus were induced
M21 0.2 0.2 100.00a Roots not consistent, long and thin, callus were induced

Table 3

Effects of culture temperature on multiplication of Scirpus × mariqueter"

Culture temperature (°C) Multiplication coefficient Differentiation status and color change of leaves
23 1.87d Yellow-green and died
25 2.68c Green, weak and thin
28 2.92abc Green
30 3.25ab Dark green, thriving
32 3.18a Dark green, few burned by high temperature

Table 6

Effects of different containers on operating efficiency of Scirpus × mariqueter"

Type of
container
Inoculation
speed (plant·h-1)
Inoculation density
(plant·container-1)
Percentage of
contamination (%)
Description of root growing
Plastic case 198.67 10 5.04a Grew strongly and good condition
Glass bottle 134.33 25 5.27a Grew strongly and good condition

Table 7

Effects of different medium and planting density on transplant and survival of Scirpus × mariqueter"

Treatment Medium and ratio (v/v) Density (plant·hole-1) No. of seedlings Survival rate (%)
1 Perlite:turf=2:1 1 60 31.67h
2 Perlite:turf=2:1 2 60 36.11gh
3 Perlite:turf=1:1 1 60 43.33fg
4 Perlite:turf=1:1 2 60 47.78ef
5 Perlite:turf=1:2 1 60 76.67b
6 Perlite:turf=1:2 2 60 69.44b
7 Perlite:turf:vermiculite=1:1:1 1 60 88.89a
8 Perlite:turf:vermiculite=1:1:1 2 60 87.22a
9 Perlite:Chongming fresh soil=1:1 1 60 58.33cd
10 Perlite:Chongming fresh soil=1:1 2 60 60.00cd
11 Chongming fresh soil 1 60 52.78de
12 Chongming fresh soil 2 60 61.67c
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