Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (6): 801-806.doi: 10.11983/CBB15220

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Effect of Paclobutrazol on Growth and Production of Esculentoside A in Hairy Roots of Phytolacca americana

Xizi Shi1, Yapeng Guo2, Heping Shi2*   

  1. 1School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
    2Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2015-12-10 Accepted:2016-06-13 Online:2016-12-02 Published:2016-11-01
  • Contact: Shi Heping
  • About author:

    # Co-first authors


To explore the possibility of the use of the plant growth regulator paclobutrazol (PP333) to regulate growth and secondary metabolism of hairy roots, we examined the effect of PP333 on the growth and production of esculentoside A in Phytolacca americana hairy roots. Compared to the control, with increasing concentration of PP333 in the medium, the lateral roots of hairy roots became short and thick and the root tips and surface of lateral roots turned light red and the root surface became redder. The addition of 0.5-5.0 mg∙L-1 PP333 in the medium promoted the production of esculentoside A in hairy roots. The highest content of esculentoside A, 6.22 mg∙g-1 dry weight, about 1.94 times that of the control, was obtained with 1.0 mg∙L-1 PP333. The enhanced production of esculentoside A by PP333 may be related to its regulation of phenylalanine ammonia lyase activities of P. americana hairy roots.

Figure 1

Effect of different concentrations of PP333 on growth and morphology of Phytolacca americana hairy roots(A) Control; (B) 0.5 mg∙L-1 PP333; (C), (D) 1.0 mg∙L-1 PP333; (E) 3.0 mg∙L-1 PP333; (F) 5.0 mg∙L-1 PP333; (G), (H) 7.0 mg∙L-1 PP333. Bar=2 cm"

Figure 2

Effect of different concentrations of PP333 in the medium on the biomass (multiplication coefficient of fresh weight) of Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

Figure 3

Effect of different concentrations of PP333 on the content of esculentoside A in Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

Figure 4

Effect of different concentrations of PP333 on the PAL (phenylalanine ammonia lyase) activity in Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

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