Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (6): 801-806.doi: 10.11983/CBB15220

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Effect of Paclobutrazol on Growth and Production of Esculentoside A in Hairy Roots of Phytolacca americana

Xizi Shi1, Yapeng Guo2, Heping Shi2*   

  1. 1School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
    2Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2015-12-10 Accepted:2016-06-13 Online:2016-12-02 Published:2016-11-01
  • Contact: Shi Heping E-mail:shihp@scnu.edu.cn
  • About author:

    # Co-first authors

Abstract:

To explore the possibility of the use of the plant growth regulator paclobutrazol (PP333) to regulate growth and secondary metabolism of hairy roots, we examined the effect of PP333 on the growth and production of esculentoside A in Phytolacca americana hairy roots. Compared to the control, with increasing concentration of PP333 in the medium, the lateral roots of hairy roots became short and thick and the root tips and surface of lateral roots turned light red and the root surface became redder. The addition of 0.5-5.0 mg∙L-1 PP333 in the medium promoted the production of esculentoside A in hairy roots. The highest content of esculentoside A, 6.22 mg∙g-1 dry weight, about 1.94 times that of the control, was obtained with 1.0 mg∙L-1 PP333. The enhanced production of esculentoside A by PP333 may be related to its regulation of phenylalanine ammonia lyase activities of P. americana hairy roots.

Figure 1

Effect of different concentrations of PP333 on growth and morphology of Phytolacca americana hairy roots(A) Control; (B) 0.5 mg∙L-1 PP333; (C), (D) 1.0 mg∙L-1 PP333; (E) 3.0 mg∙L-1 PP333; (F) 5.0 mg∙L-1 PP333; (G), (H) 7.0 mg∙L-1 PP333. Bar=2 cm"

Figure 2

Effect of different concentrations of PP333 in the medium on the biomass (multiplication coefficient of fresh weight) of Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

Figure 3

Effect of different concentrations of PP333 on the content of esculentoside A in Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

Figure 4

Effect of different concentrations of PP333 on the PAL (phenylalanine ammonia lyase) activity in Phytolacca americana hairy rootsThe different letters indicate significant differences at the 0.05 level."

[1] 陈晓光, 史春余, 王振林, 张立明, 张晓冬 (2012). 多效唑对食用甘薯北京553块根淀粉积累及相关酶活性的影响. 中国农业科学 45, 192-198.
[2] 陈晓光, 王振林, 彭佃亮, 李勇, 蔡铁, 王平, 陈二影 (2011). 种植密度与喷施多效唑对冬小麦抗倒伏能力和产量的影响. 应用生态学报 22, 1465-1470.
[3] 贾金萍, 秦雪梅, 李青山 (2003). 商陆化学成分和药理作用的研究进展. 山西医科大学学报 34, 89-92.
[4] 贾茵, 张启翔, 潘会堂, 董玲玲 (2010). PP333、CCC、B9对盆栽小报春矮化效应研究. 北京林业大学学报 32, 218-222.
[5] 赖道万, 孙文基 (2008). HPLC-ELSD法测定商陆不同部位中商陆皂苷甲的含量. 药物分析杂志 28, 1466-1469.
[6] 李明军 (1995). 多效唑——一种优良的植物生长调节剂. 植物学通报 12(2), 27-31.
[7] 林秋霞, 李敏, 周海玉, 刘聪, 任敏, 赵春艳 (2014). 植物生长调节剂对川麦冬总皂苷和总多糖含量的影响研究. 中国现代中药 16, 399-401.
[8] 罗兴录 (2003). 木薯生长、块根发育和淀粉积累化学调控及生理基础研究. 博士论文. 福州: 福建农林大学. pp. 69-73.
[9] 吕双庆, 李生秀 (2005). 多效唑对旱地小麦一些生理、生育特性及产量的影响. 植物营养与肥料学报 11, 92-98.
[10] 毛龙生, 高勇, 姚亚英, 钱遥, 唐吟岚 (1991). PP333、B9、CCC对盆栽一串红矮化效应研究. 园艺学报 18, 177-179.
[11] 乔献丽, 蒋曙光, 吕晓芬, 李凤霞, 赵德修 (2011). 激素对转基因雪莲毛状根植株再生及类黄酮产生的影响. 生物工程学报 27, 69-75.
[12] 唐湘如, 谭中文, 李之林, 余铁桥 (2002). CPPU和PP333对杂交水稻3个淀粉合成相关酶活性和米质的影响. 杂交水稻 17(3), 44-46.
[13] 万寅生, 吴项如, 李式军 (1989). 多效唑对无土育苗辣椒株型的调控. 植物生理学通讯 2, 52-54.
[14] 王敬文, 薛应龙 (1982). 植物苯丙氨酸裂解酶的研究II. 苯丙氨酸裂解酶在抗马铃薯晚疫病中的作用. 植物生理学报 8, 35-43.
[15] 翁忙玲, 吴震, 李谦盛, 刘高琼, 李式军 (2003). 多效唑(PP333)对山葵根茎膨大和内源激素含量的影响. 植物生理学通讯 39, 458-460.
[16] 翁忙玲, 吴震, 李式军 (2004). 多效唑对山葵根茎形成及营养品质的影响. 南京农业大学学报 27, 128-129.
[17] 张佳蕾, 高芳, 林英杰, 王媛媛, 杨传婷, 张凤, 李艳红, 李向东 (2013). 不同品质类型花生品质性状及相关酶活性差异. 应用生态学报 24, 481-487.
[18] 郑钦岳, 麦凯, 潘祥福, 易杨华 (1992). 商陆皂甙甲的抗炎作用. 中国药理学与毒理学杂志 6, 221-223.
[19] 朱远锋 (2013). 美洲商陆毛状根诱导和离体培养及其高产根系的筛选. 硕士论文. 广州: 华南师范大学. pp. 29-30.
[20] Arroo RRJ, Develi A, Mejers H (1995). Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures.Physiol Plant 93, 233-240.
[21] Farkya S, Bisaria VS (2008). Exogenous hormones affect- ing morphology and biosynthetic potential of hairy root line (LYR2i) of Linum album.J Biosci Bioeng 105, 140-146.
[22] Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987). New routes to plant secondary products.Biol Technol 5, 800-804.
[23] Ilan A, Dougall DK (1994). Effects of gibberellic acid and uniconazole on the activities of some enzymes of antho- cyanin biosynthesis in carrot cell cultures.J Plant Growth Regul 13, 213-219.
[24] Jaleel CA, Manivannan P, Sankar B (2007). Paclobutrazol enhances photosynthesis and ajmalicine production in Catharanthus roseus.Proc Biochem 42, 1566-1570.
[25] Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiol Plant 15, 473-497.
[26] Solecka D, Kacperska A (2003). Phenylpropanoid deficiency affects the course of plant acclimation to cold.Physiol Plant 119, 253-262.
[27] Weathers PJ, Bunk G, McCoy MC (2005). The effect of phytohormones on growth and arthmisinin production in Artemisia annua hairy roots.In Vitro Cell Dev Biol Plant 41, 47-53.
[28] White FF, Nester EW (1980). Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes.J Bacterol 141, 1134-1141.
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