Chin Bull Bot ›› 2015, Vol. 50 ›› Issue (5): 583-590.doi: 10.11983/CBB15062

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Role of PI3P and Its Relationship with H2O2 or Nitric Oxide in Darkness-induced Stomatal Closure in Vicia faba

Min Ma, Aijing Liu, Jie Hu, Junmin He*   

  1. School of Life Sciences, Shaanxi Normal University, Xi’an 710062, China
  • Received:2015-04-03 Revised:2015-05-11 Online:2015-10-09 Published:2015-09-01
  • Contact: Jun-Min He E-mail:hejm@snnu.edu.cn

Abstract:

In combination with physiological and cell biological research approaches including stomatal bioassay and laser-scanning confocal microscopy, we studied the interrelationship of phosphatidylinositol 3-phosphate (PI3P) and H2O2 or nitric oxide (NO) in darkness-induced stomatal closure in epidermal strips of Vicia faba (broad bean) by using the drug treatment with wortmannin (WM) and LY294002 (LY) to inhibit the production of PI3P. The obtained results showed that both WM and LY significantly inhibited darkness-induced synthesis of H2O2 and NO in guard cells and subsequent stomatal closure but not exogenous H2O2- and NO-induced stomatal closure. In addition, exogenous treatment with H2O2 and NO completely reversed the inhibitory effect of WM and LY on darkness-induced stomatal closure. In conclusion, our results suggest that PI3P may function upstream of H2O2 and NO in the signal transduction pathways of darkness-induced stomatal closure in V. faba.

[1]李惠民, 胡洁, 贺军民 (2013).磷脂酰肌醇3-磷酸对UV-B诱导蚕豆保卫细胞中过氧化氢产生和气孔关闭的影响.中国农业科学, 46(20), 4246-4253.
[2]Allan AC, Fluhr R (1997).Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells. The Plant Cell 9, 1559-1572.
[3]Bright J, Desikan R, Hancock JT, Weir IS, Neill SJ (2006).ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis. Plant Journal 45, 113-122.
[4]Bunney TD, Watkins PA, Beven AF, Shaw PJ, Hernandez LE, Lomonossoff GP, Shanks M, Peart J, Dr?bak BK (2000).Association of phosphatidylinositol 3-kinase with nuclear transcription sites in higher plants. The Plant Cell 12, 1679-1687.
[5]Cathcart R, Schwiers E, Ames BN (1983).Detection of picomol levels of hydroperoxides using a fluorescent dichlorifluorescein assay. Analytical Biochemistry 134, 111-116.
[6]Ellson C, Davidson K, Anderson K, Stephens LR, Hawkins PT (2006).PtdIns3P binding to the PX domain of p40phox is a physiological signal in NADPH oxidase activation. The EMBO Journal 25, 4468-4478.
[7]Foissner I, Wendehenne D, Langebartels C, Durner J (2000).In vivo imaging of an elicitor-induced nitric oxide burst in tobacco. The Plant Journal 23, 817-824.
[8]He J-M, Ma X-G, Zhang Y, Sun T-F, Xu F-F, Chen Y-P, Liu X, Yue M (2013).Role and interrelationship of Gα protein, hydrogen peroxide, and nitric oxide in ultraviolet B-induced stomatal closure in Arabidopsis leaves. Plant Physiology 161, 1570-1583.
[9]Hung KT, Kao CH (2005).Phosphatidylinositol 3-phosphate is required for abscisic acid-induced hydrogen peroxide production in rice leaves. Plant Growth Regulation 45, 95-101.
[10]Joo JH, Yoo HJ, Hwang I, Lee JS, Nam KH, Bae YS (2005).Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase. FEBS Letters 14, 1243-1248.
[11]Jung JY, Kim YW, Kwak JM, Hwang JU, Young J, Schroeder JI, Hwang I, Lee Y (2002).Phosphatidylinositol 3- and 4-phosphate are required for normal stomatal movements. The Plant Cell 14, 2399-2412.
[12]Kale SD, Gu B, Capelluto DGS, Dou D, Feldman E, Rumore A, Arredondo FD, Hanlon R, Fudal I, Rouxel T, Lawrence CB, Shan W, Tyler BM (2010).External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells. Cell 142, 284-295.
[13]Kojima H, Nakatsubo N, Kikuchi K, Urano Y, Higuchi T, Tanaka J, Kudo Y, Nagano T (1998).Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA. Neuroreport 9, 3345-3348.
[14]Kolla VA, Raghavendra AS (2007).Nitric oxide is a signaling intermediate during bicarbonate-induced stomatal closure in Pisum sativum. Physiologia Plantarum, 130(1), 91-98.
[15]Kolla VA, Vavasseur A, Raghavendra AS (2007).Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis. Planta 225, 1421-1429.
[16]Krinke O, Ruelland E, Valentová O, Vergnolle C, Renou J-P, Taconnat L.Flemr M, Burketová L, Zachowski A (2007). Phosphatidylinositol 4-kinase activation is an early response to salicylic acid in Arabidopsis suspension cells. Plant Physiology 144, 1347-1359.
[17]Lee Y, Bak G, Choi Y, Chuang WI, Cho HT, Lee Y (2008a).Roles of phosphatidylinositol 3-kinase in root hair growth. Plant Physiology 147, 624-635.
[18]Lee Y, Kim E-S, Choi Y, Hwang I, Staiger CJ, Chung Y-Y, Lee Y (2008b).The Arabidopsis phosphatidylinositol 3-kinase is important for pollen development. Plant Physiology 147, 1886-1897.
[19]Leshem Y, Seri L, Levine A (2007).Induction of phosphatidylinositol 3-kinase-mediated endocytosis by salt stress leads to intracellular production of reactive oxygen species and salt tolerance. Plant Journal 51, 185-197.
[20]Ma Y-L, She X-P, Yang S-S (2012).Sphingosine-1-phosphate (S1P) mediates darkness-induced stomatal closure through raising cytosol pH and hydrogen peroxide (H2O2) levels in guard cells in Vicia faba. Science China Life Sciences 55, 974-983.
[21]Neill S, Barros R, Bright J, Desikan R, Hancock J, Harrison J, Morris P, Ribeiro D, Wilson I (2008).Nitric oxide, stomatal closure, and abiotic stress. Journal of Experimental botany 59, 165-176.
[22]Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002).Hydrogen peroxide and nitric oxide as signalling molecules in plants. Journal of Experimental botany 53, 1237-1247.
[23]Park KY, Jung JY, Park J, Hwang JU, Kim YW, Hwang I, Lee Y (2003).A role for phosphatidylinositol 3-phosphate in abscisic acid-induced reactive oxygen species generation in guard cells. Plant Physiology 132, 92-98.
[24]Parmar PN, Brearley CA (1995).Metabolism of 3-phosphorylated and 4-phosphorylated phosphatidylinositols in stomatal guard cells of Commelina communis L. Plant Journal 8, 425-433.
[25]Peleg-Grossman S, Volpin H, Levine A (2007).Root hair curling and Rhizobium infection in Medicago truncatula are mediated by phosphatidylinositide-regulated endocytosis and reactive oxygen species. Journal of Experimental Botany 58, 1637-1649.
[26]Qiao W, Fan LM (2008).Nitric oxide signaling in plant responses to abiotic stresses. Journal of Integrative Plant Biology 50, 1238-1246.
[27]She X-P, Song X-G, He J-M (2004).Role and relationship of nitric oxide and hydrogen peroxide in light/dark-regulated stomatal movement in Vicia faba. Acta Botanica Sinica 46(11), 1292-1300.
[28]Shi CY, Qi C, Ren HY, Huang AX, Hei SM, She XP (2015).Ethylene mediates brassinosteroid-induced stomatal closure via Gα protein-activated hydrogen peroxide and nitric oxide production in Arabidopsis. Plant Journal doi: 10.1111/tpj. 12815.
[29]Thole JM, Nielsen E (2008).Phosphoinositides in plants: novel functions in membrane trafficking. Current Opinion in Plant Biology 11, 620-631.
[30]Toker A, Cantley LC (1997).Signaling through the lipid products of phosphoinositide-3-OH kinase. Nature 387, 673-676.
[31]Wang P, Song C-P (2008).Guard-cell signalling for hydrogen peroxide and abscisic acid. New Phytologist 178, 703-718.
[32]Wilson ID, Neill SJ, Hancock JT (2008).Nitric oxide synthesis and signaling in plants. Plant, Cell and Environment 31, 622-631.
[33]Zhang Y, Zhu H, Zhang Q, Li M, Yan M, Wang R, Wang L, Welti R, Zhang W, Wang X (2009).Phospholipase Dα1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis. The Plant Cell 21, 2357-2377.

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