Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (2): 251-256.doi: 10.11983/CBB15036
• TECHNIQUE AND METHOD •
Fang Liu1†, Yinghong Tang1, 2†, Youmei Yuan2, Qingquan Guo1, Fan Shen1, Jianrong Chen1*
The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L–1 6-BA+0.1 mg·L–1 NAA+1.0 mg·L–1 KT; for redifferentiation 3/4MS+3.0 mg·L–1 6-BA+0.3mg·L–1 NAA; and for rooting 1/2MS+0.03 mg·L–1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.
Fang Liu, Yinghong Tang, Youmei Yuan, Qingquan Guo, Fan Shen, Jianrong Chen. Tissue Culture of the Succulent Plant Sedum clavatum[J].Chin Bull Bot, 2016, 51(2): 251-256.
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