植物学报 ›› 2013, Vol. 48 ›› Issue (3): 295-302.doi: 10.3724/SP.J.1259.2013.00295

• 研究报告 • 上一篇    下一篇

花魔芋抗软腐病植株的鉴定及其抗性机理的初步研究

雷珍珍, 叶晶龙, 程海丽, 陈云, 望彗星, 许克静, 乐超银*   

  1. 三峡大学生物技术研究中心, 宜昌 443002
  • 收稿日期:2012-10-11 修回日期:2013-04-02 出版日期:2013-05-01 发布日期:2013-06-21
  • 通讯作者: 乐超银 E-mail:yuechaoyin@163.com
  • 基金资助:

    国家自然科学基金;湖北省自然科学基金

Characterization of Soft-rot-resistant Amorphophallus konjac and Preliminary Analysis of the Resistance Mechanism

Zhenzhen Lei, Jinglong Ye, Haili Cheng, Yun Chen, Huixing Wang, Kejing Xu, Chaoyin Yue*   

  1. Biotechnology Research Center of China Three Gorges University, Yichang 443002, China
  • Received:2012-10-11 Revised:2013-04-02 Online:2013-05-01 Published:2013-06-21
  • Contact: Chaoyin Yue E-mail:yuechaoyin@163.com
  • Supported by:

    NSFC;The natural science foundation of Hubei Province

摘要: 通过筛选获得了对魔芋(Amorphophallus konjac)软腐病具有较强抗性的花魔芋抗病植株, 经核型分析发现, 其染色体数目与普通植株一致, 均为2n=26。抗病实验结果表明, 该抗病植株对魔芋软腐病的抗性较普通植株强。采用酶联免疫吸附法, 测定了软腐病病原菌接种30小时内的抗病与普通植株叶片中内源植物激素(SA与JA)的含量变化。结果显示, 抗病和普通植株叶片中SA和JA含量变化的总体趋势明显不同。主要表现在, 抗病和普通植株叶片中SA和JA积累的时间不同; 此外, 积累的量也有明显差异。推测魔芋抗病植株可能存在与目前大多数植株不同的抗病机制。

Abstract: Amorphophallus konjac plants with strong resistance to soft-rot disease were identified from the A. konjac field in Changyang, Yichang. Karyotype analysis showed that the number of chromosomes in resistant plants were consistent with that in common A. konjac plants (susceptible), and both were 2n=26. Resistant plants were compared with susceptible A. konjac plants for disease resistance. Soft-rot-resistant plants showed stronger resistance against Erwinia carotovora var. carotovora than susceptible plants did. ELISA revealed hormone levels induced within 30 h after infection with E. carotovora. However, susceptible plants showed higher levels of jasmonate and salicylic acid than resistant plants did. These results imply a different disease resistance mechanism in resistant A. konjac plants, which can be examined by further studies.

中图分类号: 

  • Q7

白玉,杜甫佑,白玉,刘虎威 (2010). 植物激素检测技术研究进展. 生命科学 22, 36-42.
崔鸣, 谢利华, 张盛林 (2005). 不同魔芋品种产量和抗病性试验研究. 种子12, 84-85.
刘佩瑛 (2004). 魔芋学. 北京:中国农业出版社. pp. 146-174; 212-230.
刘庆安 (2010). 根结线虫侵染对黄瓜生长和内源激素含量的影响. 中国农学通报 26, 170-174.
沈业寿, 储苏 (2002). 魔芋软腐病病原菌的分离及致病性研究. 安徽大学学报 26, 96-100.
韦松基, 温标才 (2003). 桂平魔芋染色体及核型分析. 栽培与育种 26, 774.
解松峰, 宣慢, 张百忍, 刘继瑞, 马俊, 谢世学(2012). 魔芋属种质资源研究现状及应用前景. 长江蔬菜2, 7-12.
钟伏付, 苏娜, 杨廷宪, 孙正祥, 周燚 (2011). 魔芋品种选育与改良研究进展. 湖北农业科学 50, 447-448.
Devadas SK, Enyedi A, Raina R(2002). The Arabidopsis hrl1 mutation reveals novel overlapping roles for salicylic acid, jasmonic acid and ethylene signaling in cell death and defense against pathogens. Plant J 30, 467–480.
Durrant WE, Dong X (2004). Systemic acquired resistance. Ann Rev Phytopathol 42, 185–209.
Felton GW, Korth K (2000). Trade-offs between pathogen and herbivore resistance. Curr Opin Plant Biol 3, 309–314.
Heidel AJ, Baldwin IT(2004). Microarray analysis of salicylic acidand jasmonic acid signalling in responses of Nicotiana attenuate to attack by insects from multiple feeding guilds. Plant Cell Environ27, 1362-1373.
Halitschke R, Baldwin IT(2004). Jasmonates and related compounds in plant–insect interactions. J Plant Growth Regul 23, 238–245.
Hiroo Kanno, Morifumi Hasegawa, Osamu Kodama (2012). Accumulation of salicylic acid, jasmonic acid and phytoalexins in rice, Oryza sativa, infested by the white-backed plant hopper, Sogatella furcifera (Hemiptera: Delphacidae). Appl Entomol Zool 47, 27–34.
Kessler A, Baldwin IT(2002). Plant response to insect herbivory:The emerging Molecular Analysis. Annu Rev plant Boil 53, 299-328.
Kovac M, Muller A, Jarh D M (2009). Multiple hormone analysis indieated involvement of jasmonate signaling in the early defence of potato to potato virus YNTN. Brief communication 53, 195-199.
Pozo MJ, Van Loon LC, Pieterse CMJ (2004). Jasmonates: signals in plant microbe interactions. J Plant Growth Regul 23, 211-222.
Rojo E, Solano R, Sanchez-Serrano JJ (2003). Interactions between signaling compounds involved in plant defense. J Plant Growth Regul 22, 82–98.
Seo S, Seto H, Yamakawa H, Ohashi Y(2001). Transient accumulation of jasmonic acid during the synchronized hypersensitive cell death in tobacco mosaix virus-infected tobacco leaves. Mol.Plant Mierobe Illteraet 14, 261-264.
Thompson GA, Goggin FL(2006). Transcriptomics and functional genomics of plant defense induction by phloem-feeding insects. J Exp Bot 57, 755–766.
Van Loon LC(2000). Systemic induced resistance. In: Slusarenko AJ, Fraser RSS, Van Loon LC (eds) Mechanism of resistance to plant diseases. Kluwer, Dordrecht. pp. 521–574.
Van Wees MSC, de Swart EAM, van Pelt JA, van Loon LC, Pieterse CMJ(2000). Enhancement of induced disease resistance by simultaneous activation of salicylate- and jasmonate-dependent defense pathways in Arabidopsis thaliana. Proc Natl Acad Sci USA 97, 8711–8716.
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