植物学报 ›› 2019, Vol. 54 ›› Issue (2): 265-276.DOI: 10.11983/CBB18194

所属专题: 逆境生物学专辑 (2019年54卷2期)

• 专题论坛 • 上一篇    下一篇

水稻稻瘟病抗性基因的克隆、育种利用及稻瘟菌无毒基因研究进展

杨德卫1,2,王莫1,韩利波1,唐定中1,李生平1,3,*()   

  1. 1 福建农林大学植物免疫研究中心, 福州 350002
    2 福建省农业科学院水稻研究所, 福州 350018
    3 福建农林大学, 福建省作物设计育种重点实验室, 福州 350002
  • 收稿日期:2018-09-12 接受日期:2019-01-08 出版日期:2019-03-10 发布日期:2019-09-01
  • 通讯作者: 李生平
  • 基金资助:
    福建省公益项目(2017R1021-5);福建省公益项目(2017R1021-2);福建省农业科学院青年创新团队(STIT2017-3-3);福建省农业科学院科技创新项目(PC2018-2);和福建省农业科学院青年自由探索项目(NAA2018-21)

Progress of Cloning and Breeding Application of Blast Resistance Genes in Rice and Avirulence Genes in Blast Fungi

Dewei Yang1,2,Mo Wang1,Libo Han1,Dingzhong Tang1,Shengping Li1,3,*()   

  1. 1 Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2 Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China
    3 Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2018-09-12 Accepted:2019-01-08 Online:2019-03-10 Published:2019-09-01
  • Contact: Shengping Li

摘要: 稻瘟病是世界上影响水稻(Oryza sativa)粮食生产的主要病害之一, 抗病基因的发掘与利用是抗病育种的基础和核心。随着寄主水稻和病原菌稻瘟病菌(Magnaporthe oryzae)基因组测序和基因注释的完成, 水稻和稻瘟病菌的互作体系成为研究植物与真菌互作的模式系统。该文对稻瘟病抗病基因的遗传、定位、克隆及育种利用进行概述, 并通过生物信息学分析方法, 探讨了水稻全基因组中NBS-LRR类抗病基因在水稻12条染色体上的分布情况, 同时对稻瘟病菌无毒基因的鉴定及无毒蛋白与抗病蛋白的互作进行初步分析。最后对稻瘟病抗病基因研究存在的问题进行分析并展望了未来的研究方向, 以期为水稻抗稻瘟病育种发展和抗病机制的深入理解提供参考。

关键词: 稻瘟病, 基因定位, 分子克隆, 分子育种, 无毒基因

Abstract: Rice blast, caused by Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The identification and utilization of resistance genes are the basis and key factors for breeding resistance rice cultivars. With the availability of genomic sequences of both Oryza sativa and M. oryzae, rice has become one of the model systems for dissecting the molecular interactions between plants and pathogens. In this paper, we summarize the current status of genetics, mapping, cloning and breeding application of the genes resistant to blast. Using bioinformatics analysis, we analyzed the distribution of all NBS-LRR type of disease resistance genes on the 12 chromosomes of the rice genome and also preliminarily summarized the identified avirulence genes as well as interactions between the resistance proteins and the avirulence proteins. Finally, we analyzed and discussed the problems in rice breeding and prospects for research. This review will provide useful suggestions for further research on rice blast resistance breeding and disease resistance mechanisms.

Key words: rice blast, mapping, cloning, breeding application, avirulence genes