Abstract: The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L^–1 6-BA+0.1 mg·L^–1 NAA+1.0 mg·L^–1 KT; for redifferentiation 3/4MS+3.0 mg·L^–1 6-BA+0.3mg·L^–1 NAA; and for rooting 1/2MS+0.03 mg·L^–1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.