一种快速有效分析烟草花冠中花青素苷的方法

doi:10.3724/SP.J.1259.2011.00189

植物学报 ›› 2011, Vol. 46 ›› Issue (2): 189-196.DOI: 10.3724/SP.J.1259.2011.00189

一种快速有效分析烟草花冠中花青素苷的方法

孙翊¹, 李慧^2,3, 王亮生², 戴思兰^1*

¹北京林业大学园林学院, 国家花卉工程技术研究中心, 北京 100083
²中国科学院植物研究所北京植物园, 北京 100093
³中国科学院研究生院, 北京 100049

收稿日期:2010-07-16 修回日期:2011-01-28 出版日期:2011-03-01 发布日期:2011-05-09
通讯作者: 戴思兰
基金资助:
国家自然科学基金;家林业局林业公益性行业科研专项;中国科学院方向性项目

Rapid, Effective Method for Anthocyanin Analysis in Tobacco Corolla

Yi Sun¹, Hui Li^2,3, Liangsheng Wang², Silan Dai^1*

¹College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;

²Beijing Botanical Garden, Institute ofBotany, Chinese Academy of Sciences, Beijing 100093, China;

³Graduate University of Chinese Academy ofSciences, Beijing 100049, China

Received:2010-07-16 Revised:2011-01-28 Online:2011-03-01 Published:2011-05-09
Contact: Silan Dai

摘要/Abstract

摘要： 花青素苷(anthocyanins)成分和含量的分析是花色研究的重要内容之一。该文利用高效液相色谱-电喷雾离子化-质谱技术(HPLC-ESI-MS), 建立了一种快速有效地分析烟草(Nicotiana tabacum)花冠中花青素苷成分及含量的方法。在保证良好分离效果的基础上, 将分析时间缩短至15分钟, 大大提高了分析速度, 降低了成本, 并成功应用于转基因烟草花冠中花青素苷成分的分析。为不具备高效分析仪器(如超高效液相色谱-串联质谱(UPLC-MS/MS))的一般实验室研究花青素苷合成相关基因在烟草中的异源表达提供了一种有效且实用的分析方法。

Abstract: The analysis of anthocyanins is an important task for studies of flower coloration. This paper describes a rapid, effective method for analysis of anthocyanin composition and content in tobacco corolla based on high-performance liquid chromatography-electrospray ionization-mass spectrometry. HPLC analysis time was shortened to 15 min by this method, and the separation effect was guaranteed. The analysis speed is largely improved, and costs are reduced. The analysis of anthocyanins in transgenic tobacco was successful with this method, so it can be used in a general laboratory, even without advanced instruments such as ultra performance liquid chromatography-mass spectrometry/mass spectrometry. This method offers a practical approach to studies of heterologous expression of anthocyanin biosynthesis pathway- related genes in tobacco.

孙翊, 李慧, 王亮生, 戴思兰. 一种快速有效分析烟草花冠中花青素苷的方法. 植物学报, 2011, 46(2): 189-196.

Yi Sun, Hui Li, Liangsheng Wang, Silan Dai. Rapid, Effective Method for Anthocyanin Analysis in Tobacco Corolla. Chinese Bulletin of Botany, 2011, 46(2): 189-196.

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https://www.chinbullbotany.com/CN/Y2011/V46/I2/189

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一种快速有效分析烟草花冠中花青素苷的方法

Rapid, Effective Method for Anthocyanin Analysis in Tobacco Corolla

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