植物学报 ›› 2015, Vol. 50 ›› Issue (5): 591-597.DOI: 10.11983/CBB14131

• 研究报告 • 上一篇    下一篇

砷在药用植物三七根部组织及其亚细胞分布特征

陈璐1, 米艳华1,*(), 万小铭2, 袁志伟3, 尹本林1, 和丽忠1   

  1. 1云南省农业科学院质量标准与检测技术研究所, 昆明 650221
    2中国科学院地理科学与资源研究所, 北京 100101
    3昆明医学院第二附属医院, 昆明 650101
  • 收稿日期:2014-07-16 接受日期:2014-12-23 出版日期:2015-09-01 发布日期:2015-10-09
  • 通讯作者: 米艳华
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    国家自然科学基金(No.21267024)、云南省科技计划青年项目(No.2014FD063)和云南省创新人才培养计划项目(No.2014- HB059)

Distribution Characteristics of Arsenic in Medicinal Plants Panax notoginseng′s Taproots Tissue and Subcellular Components

Lu Chen1, Yanhua Mi1*, Xiaoming Wan2, Zhiwei Yuan3, Benlin Yin1, Lizhong He1   

  1. 1Agri-Food Quality Standard and Testing Technology Institute, Yunnan Academy of Agricultural Sciences, Kunming 650221, China
    2Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
    3The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
  • Received:2014-07-16 Accepted:2014-12-23 Online:2015-09-01 Published:2015-10-09
  • Contact: Mi Yanhua
  • About author:

    ? These authors contributed equally to this paper

摘要: 采用同步辐射X射线荧光分析(SRXRF)与亚细胞分布研究相结合的方法, 从细胞组织微区及亚细胞分布层面首次揭示了药用植物三七(Panax notoginseng)受砷(As)的毒害作用。研究结果表明, 三七根部的As元素多集中在表皮组织中, 且有向维管束运转的趋势; 细胞液是As主要富集的亚细胞组分, 用20 mg·L-1 As处理的三七细胞液中As含量约为不加As的200倍; 分析三七主根亚细胞组分的As含量与营养液As浓度的曲线拟合方程, 确定了营养液As浓度直接影响细胞液组分的As含量; 各组分所占比例从大到小表现为: 细胞液>细胞壁>细胞质, 20 mg·L-1 As处理的细胞液中砷含量约占三组分总量的65.78%, 达到最高比例, 细胞壁和细胞器中则始终维持较低的砷浓度。

Abstract: We used synchrotron radiation X-ray fluorescence (SRXRF) and subcellular distribution research methods to study the characteristics of arsenic (As) in roots of Panax notoginseng and subcellular distribution. Arsenic distributed on the root epidermis of P. notoginseng and had a tendency to migrate to vascular bundle. Cytoplasmic supernatant represented the mainly concentration of subcellular components, and the cytoplasmic supernatant arsenic content of 20 mg·L-1 was 200 times of CK. We analyzed the quadratic regression equation of arsenic content in subcellular constituents of P. notoginseng taproots and As concentrations in nutrient solution and found a direct relationship between As content in the subcellular constituents and the environment. The proportion of each constituent was in the order of cytoplasmic supernatant > cell wall > cytoplasmic organelles. The cytoplasmic supernatant content of 20 mg·L-1 As was the highest, accounting for about 65.78%, and cytoplasmic organelles and the cell wall always maintained low levels of As.