植物学报 ›› 2008, Vol. 25 ›› Issue (04): 491-496.

• 专题介绍 • 上一篇    下一篇

农杆菌介导单子叶植物遗传转化问题与对策

魏开发1*, 刘逸萍1, 林子英1, 杨雅芳1, 张泽宏1,贾文锁2   

  1. 1漳州师范学院生物系, 漳州 363000; 2中国农业大学农学与生物技术学院, 北京 100094
  • 收稿日期:2007-12-04 修回日期:2008-01-20 出版日期:2008-07-01 发布日期:2008-07-01
  • 通讯作者: 魏开发

Problems and Solutions in Agrobacterium tumefaciens-mediated Genetic Transformation of Monocotyledons

Kaifa Wei1*, Yiping Liu1, Ziying Lin1, Yafang Yang1, Zehong Zhang1, Wensuo Jia2   

  1. 1Department of Biotechnology, Zhangzhou Normal College, Zhangzhou 363000, China;2College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
  • Received:2007-12-04 Revised:2008-01-20 Online:2008-07-01 Published:2008-07-01
  • Contact: Kaifa Wei

摘要: 尽管十几年来农杆菌介导的单子叶植物遗传转化研究取得了较大的进步, 但仍存在着基因型限制、转化率不高和外源基因表达活性低等问题。本文综述了近几年来此项研究在感受态细胞选择与调节、预培养及共培养体系优化、转化子的筛选及外源基因表达调控等方面的主要进展。

Abstract: Many transgenic plants have been obtained through the Agrobacterium-mediated transformation for monocotyledons.However, transformation of monocotyledons plants has been constrained by factors affecting transformation efficiency such as gene type, callus induction and differentiation, and foreign gene expression. This review discusses some technological details and problems, and reviews the progresses, including selection and management of competent cells, optimization of preculture and coculture, selection, foreign gene expression and regulation.