蓝细菌Anacystis nidulans R-2 藻胆体中43 kD蛋白细胞定位的初步研究

植物学报 ›› 2005, Vol. 22 ›› Issue (03): 302-306.

蓝细菌Anacystis nidulans R-2 藻胆体中43 kD蛋白细胞定位的初步研究

李荣贵，汪靖超

(青岛大学生物系, 山东省天然色素重点实验室青岛 266071)

收稿日期:2005-01-21 修回日期:2005-03-24 出版日期:2005-06-20 发布日期:2005-06-20
通讯作者: 李荣贵 Author for correspondence. E-mail: rongguili@yahoo.com
基金资助:
国家自然科学基金项目(39525002, 39570067)资助

Preliminary Studies on the 43-kD Protein in Phycobilisomes of Cyanobacterium Anacystis nidulans R-2

LI Rong-Gui WANG Jing-Chao

(Key Laboratory for Natural Pigment of Shandong Province, Department of Biology, Qingdao University, Qingdao 266071)

Received:2005-01-21 Revised:2005-03-24 Online:2005-06-20 Published:2005-06-20
Contact: Author for correspondence. E-mail: rongguili@yahoo.com

摘要/Abstract

摘要： 高盐浓度条件下分离了蓝细菌Anacystis nidulans R-2的藻胆体, 藻胆体中存在一种43 kD的蛋白。Western blotting 分析表明, 该蛋白能与蓝细菌Fd:NADP+氧还酶中FNR结构域的抗体发生反应, 解聚的藻胆体具有FNR黄递酶的活性, 初步证明该43 kD蛋白就是Fd:NADP+氧还酶。TritonX-114分相实验表明, 这种43 kD的蛋白不能进入TritonX-114相。对藻胆体的部分解聚合实验表明, 富含外周杆的组分中不存在43 kD的蛋白。

关键词: Anacystis nidulans R-2, 藻胆体, Fd:NADP+氧化还原酶

Abstract: Phycobilisomes, containing a 43-kD protein, were isolated from Anacystis nidulans R-2. Dissociated phycobilisomes exhibited FNR diaphorase activity. Western blotting showed that the 43-kD protein could react with antibodies raised against Fd:NADP+ oxidoreductase. Analysis by Triton X-114 phase-partitioning indicated that the 43-kD protein could not enter into the detergent phase. Partial dissociation of phycobilisomes revealed no 43-kD protein in the fractions mainly containing rod components.

Key words: Anacystis nidulans R-2, Phycobilisome, Fd:NADP+ oxidoreductase

李荣贵, 汪靖超. 蓝细菌Anacystis nidulans R-2 藻胆体中43 kD蛋白细胞定位的初步研究. 植物学报, 2005, 22(03): 302-306.

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