植物学报 ›› 2010, Vol. 45 ›› Issue (06): 654-661.doi: 10.3969/j.issn.1674-3466.2010.06.002

• 研究报告 • 上一篇    下一篇

一个新的水稻叶形突变体(tll1)的遗传分析与精细定位

鞠培娜1,2, 方云霞1, 邹国兴1,3, 彭友林1, 孙川1, 胡江1, 董国军1, 曾大力1, 郭龙彪1, 张光恒1, 高振宇1, 钱前1*   

  1. 1中国水稻研究所水稻生物学国家重点实验室, 杭州 310006;
    2杭州师范大学生命与环境科学学院, 杭州 310016
    3江西省农业科学院水稻研究所, 南昌 330200
  • 收稿日期:2010-03-10 修回日期:2010-09-03 出版日期:2010-11-01 发布日期:2010-09-20
  • 通讯作者: 钱前 E-mail:qianqian188@hotmail.com

Genetic Analysis and Fine Mapping of a Novel Thread-like Leaf 1 (tll1) Mutant in Rice

Peina Ju1,2, Yunxia Fang1, Guoxing Zou1,3, Youlin Peng1, Chuan Sun1, Jiang Hu1, Guojun Dong1, Dali Zeng1, Longbiao Guo1, Guangheng Zhang1, Zhenyu Gao1, Qian Qian1*   

  1. 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;

    2College of Life and Environment Science, Hangzhou Normal University, Hangzhou 310016, China;

    3Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
  • Received:2010-03-10 Revised:2010-09-03 Online:2010-11-01 Published:2010-09-20
  • Contact: Qian Qian E-mail:qianqian188@hotmail.com

摘要: 利用甲基磺酸乙酯(ethylmethane sulphonate, EMS)诱变粳稻品种日本晴获得了一个遗传稳定的叶形突变体 thread-like leaf 1 (tll1)。该突变体在杭州表现为矮化、窄叶, 极端时仅剩主脉, 呈细丝状。将该突变体分别与籼稻品种南京6号、浙辐802和9311进行正反交配组, 遗传分析表明该突变体性状由1对隐性单基因控制。通过SSR和STS分子标记对F2代分离群体进行遗传定位, 将该基因初步定位在第12染色体SSR标记RM247和RM101之间。随后利用已公布的粳稻品种日本晴和籼稻品种9311的基因组序列, 发展了7对有多态的STS标记, 最终将该基因定位在FL13和FL14之间约94.3 kb的区间内, 为进一步克隆TLL1基因奠定了基础。

Abstract: We characterized a new dwarf mutant with thread-like leaf, designated as thread-like leaf 1 (tll1), derived from japonica cultivar Nipponbare with ethylmethane sulfonate (EMS) treatment. tll1 was crossed reciprocally with indica cultivar Nanjing 6, Zhefu 802 and 9311. Genetic analysis demonstrated that the mutant was controlled by one recessive nuclear gene. It was mapped between two simple sequence repeat makers RM247 and RM101 on chromosome 12 in rice. Seven polymorphic sequence tagged site (STS) markers were developed from the published genomic sequences of Nipponbare and 9311. Fine mapping of the TLL1 gene located it to a 94.3-kb physical region between the STS markers FL13 and FL14, which will facilitate map-based cloning and functional analysis of the gene.

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