植物学报 ›› 2010, Vol. 45 ›› Issue (04): 451-459.doi: 10.3969/j.issn.1674-3466.2010.04.008

• 研究报告 • 上一篇    下一篇

菲白竹组培苗白化、绿化突变体的超微结构及15个叶绿体编码基因的表达

袁丽钗&; 李雪平&; 彭镇华; 高健*   

  1. 国际竹藤网络中心国家林业局竹藤科学与技术重点实验室, 北京 100102
  • 收稿日期:2009-03-31 出版日期:2010-04-30 发布日期:2010-09-26
  • 通讯作者: 高健

Ultrastructure of the Albino and Green Mutants of Pleioblastus fortunei and Expression of 15 Chloroplast Genes

Lichai Yuan&; Xueping Li&; Zhenhua Peng; Jian Gao*   

  1. Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry Administration, International Center for Bamboo and Rattan, Beijing 100102, China
  • Received:2009-03-31 Online:2010-04-30 Published:2010-09-26
  • Contact: Jian Gao

摘要: 以菲白竹(Pleioblastus fortunei)组培苗绿化突变体及经60Co γ射线辐射获得的白化突变体为实验材料, 从生长特性、超微结构及15个叶绿体编码基因的拷贝数及转录水平方面进行了研究。结果显示, 白化突变体具有分化不定芽和不定根的能力, 其叶肉细胞中叶绿体缺失或结构不完整, 基因拷贝数与野生型无明显差异, 部分基因转录水平低于野生型, psbA基因的转录水平高于野生型。绿化突变体叶色一致且能稳定遗传, 与野生型绿色组织细胞的超微结构相比, 其叶绿体基粒片层数量少, 结构松散, 基因拷贝数低或相当于野生型, 多数叶绿体编码基因转录水平较野生型呈下调趋势, atpI基因转录水平高于野生型。psaA基因在两种突变体中均不表达, 仅在野生型中有微弱表达。

Abstract: The albino and green mutants of Pleioblastus fortunei were derived from 60Co γ radiation and long-term proliferation in vitro. To study the ultrastructure of the mutation and gene regulation in the aberrant chloroplasts, we investigated growth characteristics, transmission electronic microscopy data, and copy number of 15 chloroplast-encoded genes and their expression. The albino mutants showed differentiation ability of the bud and adventitious root. The chloroplasts of albino mutants were flawed or their anatomy was incomplete in mesophyll cells. Gene copy number did not differ between the albino and wild-type explants. However, transcription level of most genes was reduced in albino mutants except that the expression of psbA was increased. The color of leaves of the green mutants showed stable inheritance. The chloroplast grana lamella quantity of the green mutants was less than that of the wild type, and the anatomy in the green-sector cells was much looser than that of the wild type. The gene copy number was not higher in green mutants than in wild-type explants, and the expression of most genes, except atpI, in the green mutants seemed to decline compared with that in the wild type. The expression of atpI was higher in green mutants than in the wild type. Moreover, psaA was not expressed in the two kinds of mutants and was only weakly expressed in the wild type.

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