植物学报 ›› 2009, Vol. 44 ›› Issue (06): 725-727.DOI: 10.3969/j.issn.1674-3466.2009.06.010

• 技术方法 • 上一篇    下一篇

皱皮木瓜愈伤组织诱导与快速繁殖

范小峰, 郭小强, 肖朝霞, 刘灵霞

  

  1. 陇东学院生命科学系, 庆阳 745000
  • 收稿日期:2008-12-24 修回日期:2009-06-22 出版日期:2009-11-01 发布日期:2009-11-01
  • 通讯作者: 范小峰

Callus Induction and Rapid Propagation of Chaenomeles speciosa

Xiaofeng Fan, Xiaoqiang Guo, Zhaoxia Xiao, Lingxia Liu

  

  1. Department of Life Sciences, Longdong University, Qingyang 745000, China
  • Received:2008-12-24 Revised:2009-06-22 Online:2009-11-01 Published:2009-11-01
  • Contact: Xiaofeng Fan

摘要: 以皱皮木瓜(Chaenomeles speciosa)当年生枝条的茎尖和茎段为外植体进行离体培养与快速繁殖。结果表明: 芽萌发及愈伤组织诱导的最佳培养基是MS+2.0 mg.L-1 6-BA+1.0 mg.L-1 NAA; 愈伤组织分化最佳培养基是MS+1.0 mg.L-1 6-BA+0.05 mg.L-1 NAA; 芽增殖最佳培养基是MS+1.0 mg.L-1 6-BA+0.05-0.1 mg.L-1 NAA, 其增殖系数为6.59; 最佳生根培养基是1/2MS+1.0 mg.L-1 IAA, 生根率为86.7%; 采用珍珠岩二步移栽, 成活率可达90%以上。

Abstract: This experiment used the current stem-segment with shoot apexes and segments as explants in callus induction and rapid propagation of Chaenomeles speciosa. The optimal medium for bud and callus induction was MS+2.0 mg.L-1 6-BA+1.0mg. L-1 NAA, and the optimal propagation medium was MS+1.0 mg.L-1 6-BA+0.05-0.1 mg.L-1 NAA. The proliferation coefficient was 6.59. For callus differentiation, the optimal medium was MS+ 1.0 mg.L-1 6-BA+ 0.05 mg.L-1 NAA, and for rooting, 1/2 MS+1.0 mg. L-1 IAA. The ratio of rooting was 86.7%, and that of survival was more than 90% when small intact plants were transplanted on perlite seedbeds.