植物学报 ›› 2015, Vol. 50 ›› Issue (1): 22-31.doi: 10.3724/SP.J.1259.2015.00022

• 研究报告 • 上一篇    下一篇

eATP位于H2S下游参与乙烯诱导的拟南芥气孔关闭过程

车永梅, 张丹丹, 侯丽霞, 王兰香, 刘新*()   

  • 收稿日期:2013-11-06 接受日期:2014-04-03 出版日期:2015-01-01 发布日期:2015-04-09
  • 通讯作者: 车永梅,张丹丹,刘新 E-mail:liuxin6080@126.com
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    国家自然科学基金(No.31170237)、山东省自然科学基金(No.ZR2010CM024)和山东省泰山学者团队建设工程

Extracellular Adenosine Triphosphate Functions Downstream of Hydrogen Sulfide in Ethylene-induced Stomatal Closure in Arabidopsis thaliana

Yongmei Che, Dandan Zhang, Lixia Hou, Lanxiang Wang, Xin Liu*   

  1. Shandong Key Laboratory of Plant Biotechnology in University, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2013-11-06 Accepted:2014-04-03 Online:2015-01-01 Published:2015-04-09
  • Contact: Che Yongmei,Zhang Dandan,Liu Xin E-mail:liuxin6080@126.com
  • About author:

    ? These authors contributed equally to this paper

摘要:

以拟南芥(Arabidopsis thaliana)野生型、H2S合成突变体(Atl-cdes和Atd-cdes)和ABC转运体突变体(Atmrp4、Atmrp5 和Atmrp4/5)为材料, 探讨乙烯诱导气孔关闭过程中eATP与H2S之间的关系。结果显示, ABC转运体阻断剂格列本脲(Gli)、P2受体抑制剂磷酸吡哆醛-6-偶氮苯基-2',4'-二硫酸(PPADS)和三磷酸腺苷双磷酸酶(Apyrase)可抑制乙烯诱导的气孔关闭乙烯可提高拟南芥幼苗叶片eATP含量及AtMRP4和AtMRP5相对表达量, 但对Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量和气孔运动没有显著作用。实验结果表明, eATP是乙烯诱导拟南芥气孔关闭过程的重要信号分子, AtMRP4和AtMRP5参与胞内ATP的分泌H2S清除剂次牛磺酸(HT)能阻遏乙烯诱导的拟南芥幼苗叶片eATP含量的升高乙烯对Atl-cdes、Atd-cdes幼苗叶片eATP含量及AtMRP4和AtMRP5相对表达量无显著影响。据此推测eATP位于H2S下游参与乙烯诱导的拟南芥气孔关闭过程。

Abstract:

Using wild-type Arabidopsis, hydrogen sulfide (H2S)-synthesis mutants (Atl-cdes and Atd-cdes) and ABC- transporter mutants (Atmrp4, Atmrp5 and Atmrp4/5), we examined the effect of extracellular adenosine triphosphate (eATP) on ethylene-induced stomatal closure and its relationship with H2S. The ABC transporter blocker glibenclamide, P2 receptor inhibiter pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid and apyrase inhibited the inducing effects of ethylene on stomatal closure in Arabidopsis and ethylene-enhanced eATP content and upregulated relative expression of AtMRP4 and AtMRP5 in leaves of wild-type Arabidopsis but had no effect on eATP content or stomatal movement in Atmrp4, Atmrp5 and Atmrp4/5 mutants. Thus, eATP is involved in ethylene-induced stomatal closure, and AtMRP4 and AtMRP5 proteins take part in the secretion of intracellular ATP. The H2S scavenger hypotaurine inhibited the improving effect of ethylene on eATP content in leaves of wild-type Arabidopsis but had no effect on eATP content or relative expression of AtMRP4 and AtMRP5 in Atl-cdes and Atd-cdes mutants. eATP may be located downstream of H2S in ethylene-induced stomatal closure in Arabidopsis thaliana.

Figure 1

Gli、PPADS和Apyrase对乙烯诱导的拟南芥气孔关闭的影响 不同小写字母表示差异显著(P<0.05)"

图2

Gli对乙烯诱导拟南芥幼苗叶片eATP含量的影响"

图4

乙烯对拟南芥Atmrp4、Atmrp5和Atmrp4/5叶片气孔开度的影响 不同小写字母表示差异显著(P<0.05)"

图3

乙烯对拟南芥幼苗叶片AtMRP4和AtMRP5相对表达量的影响 不同小写字母表示差异显著(P<0.05)"

图5

乙烯对拟南芥Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量的影响 不同小写字母表示差异显著(P<0.05)"

图6

H2S清除剂HT对乙烯调控拟南芥幼苗叶片eATP含量的影响 不同小写字母表示差异显著(P<0.05)"

图7

乙烯对拟南芥Atl-cdes和Atd-cdes幼苗叶片eATP含量的影响 不同小写字母表示差异显著(P<0.05)"

图8

H2S对拟南芥Atmrp4、Atmrp5和Atmrp4/5突变体幼苗叶片eATP含量的影响 不同小写字母表示差异显著(P<0.05)"

图9

H2S对拟南芥叶片AtMRP4(A)和AtMRP5(B)相对表达量的影响 不同小写字母表示差异显著(P<0.05)"

图10

乙烯对拟南芥Atl-cdes (A, B)和Atd-cdes (C, D)叶片AtMRP4和AtMRP5基因表达量的影响 不同小写字母表示差异显著(P<0.05)"

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