非洲菊微管相关蛋白基因GMAP65-1功能分析

doi:10.3724/SP.J.1259.2015.00012

植物学报 ›› 2015, Vol. 50 ›› Issue (1): 12-21.DOI: 10.3724/SP.J.1259.2015.00012

非洲菊微管相关蛋白基因GMAP65-1功能分析

李凌飞, 彭建宗, 王小菁^*

华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州 510631

收稿日期:2014-03-14 修回日期:2014-11-01 出版日期:2015-01-01 发布日期:2015-04-09
通讯作者: wangxj@scnu.edu.cn
基金资助:
国家自然科学基金(No.31372099)、教育部高等学校博士学科点专项科研基金(No.20104407110005)和广东省自然科学基金(No.9251063101000002)

Preliminary Functional Analysis of Microtubule-associated Protein GMAP65-1 from Gerbera hybrida

Lingfei Li, Jianzong Peng, Xiaojing Wang^*

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China

Received:2014-03-14 Revised:2014-11-01 Online:2015-01-01 Published:2015-04-09

摘要/Abstract

摘要： 微管相关蛋白在植物生长发育过程中发挥重要作用。利用反转录聚合酶链式反应(RT-PCR)和快速扩增cDNA末端(RACE)技术对非洲菊(Gerbera hybrida)体内微管相关蛋白GMAP65-1基因进行了克隆, 获得的基因全长1 883 bp, 包含 1 740 bp的完整开放阅读框(ORF)。表达模式研究表明, 该基因在非洲菊幼嫩的根、叶及花中均有较高的表达, 且受到赤霉素(GA)诱导显著上调。构建GMAP65-1超表达载体, 经异源转化拟南芥(Arabidopsis thaliana)后筛选获得纯合株系, 对纯合株系进行表型观察。结果表明, GMAP65-1超表达植株的叶片及花瓣面积增大, 暗示该基因参与叶片及花瓣的形态建成。研究结果为花卉分子育种提供了理论依据及基因资源。

Abstract: Microtubule-associated proteins play vital roles in plant growth and development. We cloned a full cDNA encoding microtubule-associated protein GMAP65-1 by RT-PCR and RACE techniques and characterized the clone. The complete cDNA (1 883 bp) contains a 1 740-bp open reading frame encoding 579 amino acid residues. The gene expression of GMAP65-1 was higher in young roots, leaves and ray florets. Moreover, its expression was induced by gibberellin. Overexpression of GMAP65-1 in transgenic Arabidopsis enlarged the area of leaf and petal. GMAP65-1 might be involved in leaf and petal morphogenesis. This study contributes to providing the theoretical basis and gene resources for flower molecular breeding of Gerbera.

李凌飞, 彭建宗, 王小菁. 非洲菊微管相关蛋白基因GMAP65-1功能分析. 植物学报, 2015, 50(1): 12-21.

Lingfei Li, Jianzong Peng, Xiaojing Wang. Preliminary Functional Analysis of Microtubule-associated Protein GMAP65-1 from Gerbera hybrida. Chinese Bulletin of Botany, 2015, 50(1): 12-21.

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非洲菊微管相关蛋白基因GMAP65-1功能分析

Preliminary Functional Analysis of Microtubule-associated Protein GMAP65-1 from Gerbera hybrida

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