植物学报 ›› 2013, Vol. 48 ›› Issue (2): 145-150.doi: 10.3724/SP.J.1259.2013.00145

• 研究报告 • 上一篇    下一篇

红花玉兰MwAG基因在花发育不同时期的表达

景丹龙1, 马江1, 张博1, 韩逸洋1, 刘志雄2, 陈发菊1*   

  1. 1三峡大学生物技术研究中心, 宜昌 443002;
    2长江大学园艺园林学院, 荆州 434025
  • 收稿日期:2012-09-12 修回日期:2012-12-26 出版日期:2013-03-01 发布日期:2013-04-07
  • 通讯作者: 陈发菊 E-mail:chenfj616@163.com
  • 基金资助:

    国家自然基金面上项目

Expression Analysis of MwAG in Different Organs and Developmental Stages of Magnolia wufengensis

Danlong Jing1, Jiang Ma1, Bo Zhang1, Yiyang Han1, Zhixiong Liu2, Faju Chen1*   

  1. 1Biotechnology Research Center, China Three Gorges University, Yichang 443002, China;

    2College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
  • Received:2012-09-12 Revised:2012-12-26 Online:2013-03-01 Published:2013-04-07
  • Contact: Faju Chen E-mail:chenfj616@163.com

摘要: MwAG基因是调控红花玉兰(Magnolia wufengensis)雌雄蕊发育的关键转录因子。采用半定量RT-PCR、Northern blot杂交和实时荧光定量PCR技术检测了MwAG基因在红花玉兰花芽形态分化几个关键时期表达的组织特异性和表达水平的变化。研究结果表明, MwAG基因仅在红花玉兰雌雄蕊中表达, 而在幼叶、外轮花被和内轮花被中不表达。在花器官形态分化过程中, MwAG基因在雌雄蕊原基分化期和雌雄蕊成熟期均维持在一个较高的水平, 且在雄蕊中的表达波峰早于雌蕊, 这与雌雄蕊形态分化的时间基本吻合; 在花芽分化早期, 相同大小的花芽, 瓣数越多, MwAG基因在雌雄蕊中的表达量越低, 其结果与不同瓣数雌雄蕊分化的时间一致, 即瓣数越多, 雌雄蕊分化越晚。

Abstract: The function gene MwAG as a key transcription factor that plays crucial roles in the development of Magnolia wufengensis by regulating the development of the stamen and carpel. Semi-quantitative RT-PCR, Northern blot and quantitative RT-PCR methods were used to identify the spatial and temporal expression patterns of MwAG in M. wufengensis at several important stages of the morphological differentiation of flower buds. MwAG was expressed specially in the stamen and pistil, with no detectable expression in outer and inner whorls of the perianth and leaves. The expression was highest at the primordium and mature stages of stamen and pistil during morphological differentiation of organs. The expression peaked earlier in the stamen than in the pistil, and the time of expression was the same as with morphological differentiation of the stamen and pistil. At the early stage of the differentiation of flower buds, the expression was low when flower buds had more petal numbers of the same size, and the time of expression was consistent with the development and differentiation of the stamen and pistil in different flower buds (i.e., the more the petal numbers, the later the time of differentiation of stamen and pistil). MwAG may play a crucial role in stamen and carpel development of M. wufengensis.

贺随超,马履一,陈发菊 (2007). 红花玉兰种质资源遗传多样性初探[J]. 西北植物学报, 27(12): 2421-2428.
贺随超,马履一 (2008). 红花玉兰与玉兰亚属几个种亲缘关系的AFLP分析[J]. 植物研究, 28(3): 288-292.
王晓玲,马履一,贾忠奎,朱仲龙,张田田 (2011). 红花玉兰研究进展[J]. 北方园艺, (16): 202-205.
王罗荣, 马履一, 王希群, 贺随超, 刘鑫 (2007). 红花玉兰播种育苗技术的初步研究[J]. 浙江林学院学报, 24(2): 242-246.
贺窑青,马履一, 桑子阳 (2010). 红花玉兰花色形成的初步研究[J]. 西北植物学报, 30(11): 2252-2257.
曾英, 胡金勇, 李志坚 (2011). 植物MADS盒基因与花器官的进化发育[J]. 植物生理学通讯, 37(4): 281-287.
Coen ES, Meyerowitz EM (1991). The war of the whorls: genetic inter actions controlling flower development[J]. Nature, 353: 31-37.
Richard G.H. Immink, Kerstin Kaufmann, Gerco C. Angenent (2010). The ‘ABC’ of MADS domain protein behaviour and interactions[J]. Seminars in Cell & Developmental Biology, 21: 87-93.
Wentao Wu, Faju Chen, Danlong Jing, Zhixiong Liu, Lvyi Ma (2012). Isolation and Characterization of an AGAMOUS-Like Gene from Magnolia wufengensis(Magn oliaceae)[J]. Plant Mol Biol Rep, 30: 690-698.
Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2—△△Ct method. Methods, 25(4): 402-408.
Mark P. Running, Elliot M. Meyerowitz (1996). Mutations in the PERIANTHIA gene of Arabidopsis specifically alter floral organ number and initiation pattern[J]. Development, 122: 1261-1269.
Ying Wang, Xiaomei Zhang, Zhixiong Liu, Dandan Zhang, Jinzi Wang, Di Liu , Fenglan Li, Hai Lu (2012). Isolation and characterization of an AGAMOUS -like gene from Hosta plantaginea[J]. Mol Biol Rep , 39, (3): 2875-2881.
Annick Dubois, Olivier Raymond, Marion Maene, Sylvie Baudino, Nicolas B. Langlade, Ve′roniqueBoltz, Philippe Vergne, Mohammed Bendahmane (2010). Tinkering with the C-Function: A Molecular Frame for the Selection of Double Flowers in Cultivated Roses[J]. PLoS ONE, 5(2): e9288.
Kelsey D. Galimba, Theadora R. Tolkin, Alessandra M. Sullivan, Rainer Melzer, Günter Thei?en,and Verónica S. Di Stilio (2012). Loss of deeply conserved C-class floral homeotic gene function and C- and E-class protein interaction in a double-flowered ranunculid mutant. Proc Natl Acad Sci U S A. 109,(34):E2267-75.
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