植物学报 ›› 2013, Vol. 48 ›› Issue (1): 34-41.doi: 10.3724/SP.J.1259.2013.00034

• 研究报告 • 上一篇    下一篇

小麦中编码未知蛋白的热胁迫响应基因TaWTF1的克隆和功能分析

秦丹丹1,2, 谢颂朝2, 刘刚2, 倪中福2, 姚颖垠2, 孙其信2,3, 彭惠茹2*   

  1. 1湖北省农业科学院粮食作物研究所, 武汉 430064;
    2中国农业大学, 北京 100193
    3西北农林科技大学, 杨陵 712100
  • 收稿日期:2012-05-22 修回日期:2012-07-26 出版日期:2013-01-01 发布日期:2012-11-01
  • 通讯作者: 彭惠茹 E-mail:penghuiru@cau.edu.cn
  • 基金资助:

    高温诱导的小麦TaGAST基因的功能鉴定及其调控机理研究;小麦抗旱耐高温相关转录因子家族基因克隆及重要候选基因功能鉴定;小麦热胁迫响应基因TaUCH1的克隆和功能鉴定

Isolation and Functional Characterization of Heat-stressresponsive Gene TaWTF1 from Wheat

Dandan Qin1,2, Songchao Xie2, Gang Liu2, Zhongfu Ni2, Yingyin Yao2, Qixin Sun2,3, Huiru Peng2*   

  1. 1Institute of Food Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China;

    2China Agricultural University, Beijing 100193, China;

    3Northwest Agriculture and Forest University, Yangling 712100, China
  • Received:2012-05-22 Revised:2012-07-26 Online:2013-01-01 Published:2012-11-01
  • Contact: Huiru Peng E-mail:penghuiru@cau.edu.cn

摘要: WTF1 (What’s this factor 1)是包含“Domain of Unknown Function 860”(DUF860)的一类蛋白, 特异定位于植物细胞叶绿体或线粒体中, 在内含子的剪切中发挥作用。在研究小麦(Triticum aestivum)热胁迫转录谱时发现一个包含该结构域的探针受高温诱导表达。通过对其所编码的基因TaWTF1进行克隆并详细分析, 发现该基因的启动子区包含HSE、干旱、GA及SA等胁迫和激素响应元件, 且该基因在苗期和开花期均受热胁迫诱导表达。在开花期, TaWTF1在普通叶中的表达显著高于包括旗叶在内的其它组织器官。进一步将该基因在拟南芥(Arabidopsis thaliana)中超表达, 显著提高了转基因植株在热胁迫下的成活率, 说明TaWTF1参与了植物耐热性。该研究为解析植物耐热性分子机理开辟了新的领域, 并为作物耐热性分子育种提供了候选基因。

Abstract: What’s this factor 1 genes (WTF1s) encode a group of proteins with Domain of Unknown Function 860 which are localized specifically in the plant chloroplast and mitochondria and play an important role in intron splicing. Previously, a heat-responsive probe with this conserved domain was identified in the Affymetrix Wheat Genome Array. With the probe sequence, the open reading frame was cloned from wheat and was named TaWTF1. Bioinformatics analysis showed that the promoter of TaWTF1 contained some stress- and hormone-responsive elements including a heat-shock element. TaWTF1 gene expression was induced by heat treatments at the seedling and flowering stages. Moreover, the expression of this gene was higher in the common leaves. Importantly, overexpression of TaWTF1 improved the survival rate of Arabidopsis under heat stress. This study provides valuable information to understand more about the molecular mechanism of thermotolerance in plants.

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