植物学报 ›› 2011, Vol. 46 ›› Issue (1): 67-73.doi: 10.3724/SP.J.1259.2011.00067

• 技术方法 • 上一篇    下一篇

水稻蛋白质组双向电泳优化流程及方法

丁承强, 马丹, 王绍华, 丁艳锋*   

  1. 南京农业大学农学院, 南京 210095
  • 收稿日期:2010-09-20 修回日期:2010-11-05 出版日期:2011-01-01 发布日期:2011-01-20
  • 通讯作者: 丁艳锋 E-mail:dingyf@njau.edu.cn
  • 基金资助:

    水稻穗粒数基因型差异的生理生化机理和氮素调控原理;国家科技支撑计划

Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics

Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding*   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-09-20 Revised:2010-11-05 Online:2011-01-01 Published:2011-01-20
  • Contact: Yanfeng Ding E-mail:dingyf@njau.edu.cn

摘要: 双向电泳是分析蛋白质混合物的一种有力手段, 已在蛋白质组研究中得到广泛应用。水稻(Oryza sativa)作为重要的粮食作物, 对其蛋白质组学研究开展较早。但由于技术复杂, 对实验操作要求高, 初学的研究者很难在较短的时间内掌握该实验技术。该文介绍了水稻研究中适合多个组织的双向电泳实验方法和优化流程。该优化流程能使新的研究者逐步优化实验条件, 更快更好地完成双向电泳实验。同时详细介绍了实验关键环节的操作方法。

Abstract: 2-D electrophoresis is a powerful tool for analyzing protein mixtures. It has been widely used in proteome research. Rice is an important food crop, and proteomic research into rice has been extensive. Because of the technical complexity and time constraints, performing the procedure accurately has been difficult. This paper describes an optimization process for 2-D electrophoresis of various organs of rice. This method can help researchers optimize experimental conditions with better accuracy.

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