东乡野生稻苗期耐冷性的遗传分析

doi:10.3724/SP.J.1259.2011.00021

植物学报 ›› 2011, Vol. 46 ›› Issue (1): 21-27.DOI: 10.3724/SP.J.1259.2011.00021

东乡野生稻苗期耐冷性的遗传分析

简水溶^1,3, 万勇², 罗向东¹, 方军³, 储成才³, 谢建坤^1,2*

¹江西师范大学生命科学学院, 南昌 330022;
²江西省农业科学院水稻研究所, 南昌 300200
³中国科学院遗传与发育生物学研究所, 北京 100101

收稿日期:2010-07-27 修回日期:2010-09-24 出版日期:2011-01-01 发布日期:2011-01-20
通讯作者: 谢建坤
基金资助:
教育部留学回国人员科研启动基金资助项目, 江西省教育厅重点项目;国家自然基金项目;江西师范大学研究生创新基金项目

Genetic Analysis of Cold Tolerance at the Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon)

Shuirong Jian^1,3, Yong Wan², Xiangdong Luo¹, Jun Fang³, Chengcai Chu³, Jiankun Xie^1,2*

¹College of Life Science, Jiangxi Normal University, Nanchang 330022, China;

²Rice Research Institute, Jiangxi Academy ofAgricultural Science, Nanchang 300200, China;

³Institute of Genetics and Developmental Biology, Chinese Academy ofSciences, Beijing 100101, China

Received:2010-07-27 Revised:2010-09-24 Online:2011-01-01 Published:2011-01-20
Contact: Jiankun Xie

摘要/Abstract

摘要： 以强耐冷材料龙稻5号(Oryza sativa ssp. japonica)和冷敏感材料协青早B(O. sativa ssp. indica)为对照品种, 以萎蔫率和死苗率为鉴定指标, 利用江西东乡普通野生稻(O. rufipogon)/协青早B//协青早B构建的回交重组自交系(backcross inbred lines, BILs)群体(BC₁F₉)进行苗期耐冷性鉴定和遗传分析。结果表明, 10°C冷处理7天后, 228个BILs群体的平均萎蔫率为67.4%, 平均死苗率为70.8%。将死苗率≤20%的株系, 在更低温度(8°C昼/5°C夜)下处理5天, 结果显示5243和5335为强耐冷材料, 可用于构建东乡野生稻QTL(quantitative trait loci)近等基因系(near-isogenic lines, NILs)。实验结果显示, 群体萎蔫率和死苗率均呈偏态的连续分布, 暗示东乡野生稻苗期耐冷性表现为质量-数量性状遗传特征, 由主基因-多基因控制。

Abstract: We examined the cold tolerance of seedlings of a BILs population (BC₁F₉) of Dongxiang wild rice (Dwr)/Xieqingzao B (Xqz B)//Xqz B by cold treatment assay and genetic analysis. The control materials included the strong cold-tolerant variety, Longdao 5, and the cold-sensitive cultivar Xqz B, using seedling wilting rate and seedling mortality rate as evaluation indices. After cold treatment (10°C) for 7 days, the mean seedling wilting rate and seedling mortality rate for 228 BILs were 67.4% and 70.8%, respectively. The lines with only 20% seedling mortality rate or below were further treated with a colder temperature of 8°C day/5°C night for 5 days. The lines 5243 and 5335 were the most coldtolerant and may be useful for constructing quantitative trait loci-near-isogenic lines of Dwr. Moreover, the skew-normal and continuous distribution of seedling wilting and mortality rates suggest that cold tolerance at the seedling stage in Dwr represents the inheritance of qualitative-quantitative traits which are controlled by major genes and polygenes.

中图分类号:

简水溶, 万勇, 罗向东, 方军, 储成才, 谢建坤. 东乡野生稻苗期耐冷性的遗传分析. 植物学报, 2011, 46(1): 21-27.

Shuirong Jian, Yong Wan, Xiangdong Luo, Jun Fang, Chengcai Chu, Jiankun Xie. Genetic Analysis of Cold Tolerance at the Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon). Chinese Bulletin of Botany, 2011, 46(1): 21-27.

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https://www.chinbullbotany.com/CN/Y2011/V46/I1/21

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东乡野生稻苗期耐冷性的遗传分析

Genetic Analysis of Cold Tolerance at the Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon)

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