植物紫色酸性磷酸酶基因家族功能研究进展

doi:10.11983/CBB18044

摘要/Abstract

摘要： 紫色酸性磷酸酶(PAPs)是一类广泛存在于植物体内的金属磷酸酯酶, 其羧基端含有1个保守结构域, 由5个保守基序和7个氨基酸残基构成。作为一种特殊的酸性磷酸酶, PAPs在酸性环境下能够有效催化磷酸酯或酸酐的水解, 释放出植物可以利用的磷酸基团。此外, PAPs在调节植物碳代谢、细胞壁合成和抵御病菌侵染等方面也发挥重要生理作用。该文简要介绍了PAPs的结构、家族成员及其调控因子, 并着重总结了近年来对PAPs生物学功能的研究进展, 为今后系统开展PAPs功能研究提供了理论参考。

关键词: 紫色酸性磷酸酶, 生物学功能, 调控因子

Abstract: Purple acid phosphatases (PAPs) are members of the metallo-phosphoesterase family identified from a wide range of plants. They are characterized by the presence of five conserved structure motifs and seven amino acid residues in the C-terminal. PAPs have mostly been studied for their potential involvement in phosphorus acquisition and redistribution because of their ability to catalyze the hydrolysis of activated phosphate esters and anhydrides under acidic conditions. Recent studies also showed that PAPs play important roles in modulating plant carbon metabolism, cell wall synthesis and pathogen resistance, etc. This review focuses on the structure, family members and regulatory factors of PAPs, with special emphasis on the recent progress of their biological functions, which will provide theoretical reference for further study of PAPs in plants.

Key words: purple acid phosphatases (PAPs), biological function, regulatory factors

魏铭,王鑫伟,陈博,宋程威,杜亮,肖建伟,林金星. 植物紫色酸性磷酸酶基因家族功能研究进展. 植物学报, 2019, 54(1): 93-101.

Ming Wei,Xinwei Wang,Bo Chen,Chengwei Song,Liang Du,Jianwei Xiao,Jinxing Lin. Research Progress into the Function of Purple Acid Phosphatase Gene Family in Plants. Chinese Bulletin of Botany, 2019, 54(1): 93-101.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB18044

https://www.chinbullbotany.com/CN/Y2019/V54/I1/93

图/表 4

图1 菜豆紫色酸性磷酸酶(KBPAP)的三维结构(改自Strater et al., 1995)

Figure 1 3D-structure of KBPAP of Phaseolus vulgaris (modified from Strater et al., 1995)

图2 植物PAPs物种间的进化关系(改自Tian and Liao, 2015) At: 拟南芥; Os: 水稻; Gm: 大豆; Mt: 蒺藜苜蓿; Nt: 烟草; Zm: 玉米; Hv: 大麦; Ta: 小麦; Vr: 绿豆; Pv: 菜豆

Figure 2 Phylogenetic relationships of plant PAPs (modified from Tian and Liao, 2015) At: Arabidopsis thaliana; Os: Oryza sativa; Gm: Glycine max; Mt: Medicago truncatula; Nt: Nicotiana tabacum; Zm: Zea mays; Hv: Hordeum vulgare; Ta: Triticum aestivum; Vr: Vigna radiata; Pv: Phaseolus vulgaris

表1 植物PAP的生物学特性和功能(改自Tian and Liao, 2015)

Table 1 Biochemical properties functions of plant PAPs (modified from Tian and Liao, 2015)

蛋白	物种	功能	参考文献
AtPAP2	拟南芥	碳代谢	Sun et al., 2012a
AtPAP10	拟南芥	参与初生壁合成	Kaida et al., 2003
AtPAP12	拟南芥	磷代谢	Wang et al., 2014
AtPAP15	拟南芥	响应非生物逆境胁迫	Zhang et al., 2008
AtPAP25	拟南芥	磷代谢	Del Vecchio et al., 2014
AtPAP26	拟南芥	磷代谢	Wang et al., 2014
GmPAP3	大豆	响应非生物逆境胁迫	Li et al., 2008
GmPAP4	大豆	响应非生物逆境胁迫	Kong et al., 2014
NtPAP12	烟草	参与初生壁合成	Kaida et al., 2010
PvPAP3	菜豆	磷代谢	Liang et al., 2010
StPAP1	马铃薯	磷代谢	Zimmermann et al., 2004

图3 缺磷情况下分泌型酸性磷酸酶的诱导表达及其根际分泌与排根形成示意图(改自Wasaki et al., 2003) (A) 磷充足条件下, SAP聚集在根表皮细胞周围, 只有少量SAP分泌; (B) 磷胁迫条件下, SAP在12小时内从根部迅速分泌, 但在长时间保持较低水平; (C) 组织中磷含量明显下降后, 根系成簇排列, 形成排根, SAP分泌量显著增加。

Figure 3 Schematic model of SAP expression and formation of cluster root under phosphorus deficiency (modified from Wasaki et al., 2003) (A) Under sufficient P conditions, SAP localizes around the epidermal cells of roots, and only a small amount of SAP is secreted; (B) After P stress treatment, SAP is released from roots rapidly within 12 h, however, SAP secretion remains at a low level for a long time; (C) After the decrease of P content in tissues, cluster roots form and SAP secretion significantly increases.

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