植物学报 ›› 2017, Vol. 52 ›› Issue (5): 539-542.doi: 10.11983/CBB17126

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突破复杂性状多基因转化技术壁垒, 首创胚乳花青素 高积累的水稻新种质

朱丽, 钱前*()   

  1. 中国水稻研究所, 水稻生物学国家重点实验室, 杭州 311401
  • 收稿日期:2017-07-03 接受日期:2017-08-30 出版日期:2017-09-01 发布日期:2017-07-10
  • 通讯作者: 钱前 E-mail:qianqian188@hotmail.com

Development of “Purple Endosperm Rice” by Engineering Anthocyanin Biosynthesis in Endosperm: Significant Breakthrough in Transgene Stacking System, New Progress in Rice Biofortification

Li Zhu, Qian Qian*()   

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311401, China
  • Received:2017-07-03 Accepted:2017-08-30 Online:2017-09-01 Published:2017-07-10
  • Contact: Qian Qian E-mail:qianqian188@hotmail.com

摘要:

随着转基因技术的日趋成熟, 利用生物工程手段加快改良作物农艺性状, 已经越来越显示出其巨大的应用潜力。在改良多基因调控的复杂农艺性状方面, 单基因转化收效甚微, 而长期以来多基因转化不仅受限于技术因素, 而且在协调表达调控、代谢及修饰等一系列相关基因方面更是难于突破。近期, 我国科学家首次利用自创的多基因垛叠表达系统, 成功在水稻(Oryza sativa)胚乳中合成了具有抗氧化活性的花青素, 在复杂性状多基因转化领域取得了突破性进展。

关键词: 花青素, 多基因转化, 胚乳, 无选择标记, 水稻

Abstract:

With improved transgenic technology, there is great potential for bio-fortification of crops. For complex agronomic traits controlled by multiple genes, single gene transformation is insufficient, and multi-gene engineering is limited to technical factors. Regulation and expression of metabolic modification and a series of related genes is more difficult to break through. Recently, Chinese scientists successfully engineered sophisticated anthocyanin biosynthesis in rice endosperm, which suggests the potential utility of the TransGene Stacking II System for synthetic biology and improving agronomic traits in crops.

Key words: anthocyanin, multiple gene transformation, endosperm, marker excision, rice

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