植物学报 ›› 2017, Vol. 52 ›› Issue (5): 622-630.DOI: 10.11983/CBB16116

• 技术方法 • 上一篇    下一篇

火龙果果皮中可溶性膳食纤维的提取方法

张桂春, 刘玉静, 李延敏, 牟萍, 曲明娟, 李清, 周菊华*()   

  1. 鲁东大学生命科学学院/鲁东大学肿瘤免疫新技术研究中心, 烟台 264025
  • 收稿日期:2016-05-23 接受日期:2017-01-10 出版日期:2017-09-01 发布日期:2017-07-10
  • 通讯作者: 周菊华
  • 基金资助:
    山东省“泰山学者”建设工程专项(No.tshw20120718)

Extraction of Soluble Dietary Fibers from Pitaya Peel

Guichun Zhang, Yujing Liu, Yanmin Li, Ping Mu, Mingjuan Qu, Qing Li, Juhua Zhou*   

  1. School of Life Sciences, Ludong University/Research Center of New Technology for Tumor Immunology, Ludong University, Yantai 264025, China
  • Received:2016-05-23 Accepted:2017-01-10 Online:2017-09-01 Published:2017-07-10
  • Contact: Juhua Zhou

摘要: 火龙果(Hylocereus undulatus)果皮可以作为一种优良的膳食纤维来源, 且其膳食纤维具有良好的理化性能。为提高火龙果果皮的综合利用水平, 该研究以火龙果果皮为原料, 首先采用纤维素酶水解法对火龙果果皮中的可溶性膳食纤维进行提取, 然后采用单因素试验和响应面法优化酶提取工艺。结果表明, 纤维素酶法提取火龙果果皮中可溶性膳食纤维的优化工艺条件为: 纤维素酶浓度0.54%, 酶解温度50°C, pH5.2。在此条件下, 可溶性膳食纤维的提取率可达19.81%; 膳食纤维的持水力为31.25 g∙g-1, 溶胀性为29.11 mL∙g-1

关键词: 火龙果果皮, 可溶性膳食纤维, 纤维素酶, 响应面法

Abstract: Pitaya peel can be a good source for preparing dietary fibers, and such dietary fibers has excellent physicochemical properties. To stimulate the comprehensive utilization of pitaya, we optimized the extraction of soluble dietary fibers (SDF) from pitaya peel. The cellulase digestion method was used with single-factor experiments and the response surface method. The optimized extraction conditions were cellulase concentration of 0.54%, enzymolysis temperature at 50°C and solvent pH of 5.2. Under the optimal conditions, the extraction rate of SDF from pitaya peel was up to 19.81%, the water holding capacity was 31.25 g∙g-1 and the swelling capacity was 29.11 mL∙g-1.

Key words: Pitaya peel, soluble dietary fibers, cellulase, response surface method