植物学报 ›› 2016, Vol. 51 ›› Issue (6): 764-773.doi: 10.11983/CBB16004

• 研究报告 • 上一篇    下一篇

转辽宁碱蓬SlNAC4拟南芥差异表达基因分析

李倩倩, 焦杨, 于静洋, 李秋莉*   

  1. 辽宁师范大学生命科学学院/辽宁省植物生物技术重点实验室, 大连 116081
  • 收稿日期:2016-01-08 修回日期:2016-06-15 出版日期:2016-11-01 发布日期:2016-12-02
  • 通讯作者: 李秋莉 E-mail:skyliqiuli@163.com
  • 基金资助:

    国家自然科学基金(No.31340052)

Analysis of Differentially Expressed Genes in Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis

Qianqian Li, Yang Jiao, Jingyang Yu, Qiuli Li*   

  1. College of Life Sciences, Liaoning Normal University/Key Laboratory of Plant Biotechnology of Liaoning Province, Dalian 116081, China
  • Received:2016-01-08 Revised:2016-06-15 Online:2016-11-01 Published:2016-12-02
  • Contact: Qiuli Li E-mail:skyliqiuli@163.com

摘要:

以实验室前期获得的转SlNAC4基因拟南芥(Arabidopsis thaliana)和野生型拟南芥为材料, 通过基因芯片技术检测其基因表达谱。结果表明, 与野生型拟南芥相比, 转SlNAC4基因拟南芥中差异表达的基因共有3 094个。 通过GO分析, 发现与非生物胁迫相关的差异基因共有195个, 与生长发育相关的差异基因共有47个, 其中包含MYB和WRKY转录因子基因。KEGG分析表明, 差异表达基因主要涉及植物激素信号转导和油菜素内酯合成等信号通路。进一步选择部分差异表达基因进行实时荧光定量PCR分析, 所得结果与芯片检测结果一致。该研究结果表明, SlNAC4可直接或间接地调控多个下游基因的表达, 进而调控植物的生长发育, 提高其抗逆性。

Abstract:

Microarray assay was used for gene expression profiling of Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis and the wild type. SlNAC4-transgenic Arabidopsis showed 3 094 differentially expressed genes as compared with the wild type. Gene ontology analysis revealed 195 differentially expressed genes related to abiotic stress and 47 related to growth and development, including MYB and WRKY transcription factor genes. KEGG analysis revealed the differentially expressed genes involved in 90 signaling pathways. The main signaling pathways were plant hormone signal transduction and brassinosteroid synthesis. The differential expression of selected genes was validated by qRT-PCR. SlNAC4 regulated the expression of downstream genes directly and indirectly, functioning in plant growth and development and abiotic stress tolerance.

中图分类号: 

  • Q945.78

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