拟南芥短肽激素PROPEP基因家族在根生长中的作用机理

doi:10.11983/CBB15088

植物学报 ›› 2016, Vol. 51 ›› Issue (2): 202-209.DOI: 10.11983/CBB15088

拟南芥短肽激素PROPEP基因家族在根生长中的作用机理

李冬梅¹, 王路雅¹ , 张澜玥² , 帖子阳² , 毛惠平^1*

¹内蒙古大学, 呼和浩特 010021;
²内蒙古农业大学附属小学, 呼和浩特 010018

收稿日期:2015-05-18 修回日期:2015-08-05 出版日期:2016-03-01 发布日期:2016-03-31
通讯作者: 毛惠平
基金资助:
内蒙古自然科学基金(No.2013MS0507)

Mechanism of Arabidopsis Short Peptide Hormones PROPEP Gene Family in the Root Growth

Dongmei Li¹, Luya Wang¹, Lanyue Zhang², Ziyang Tie², Huiping Mao^1*

¹Inner Mongolia University, Hohhot 010021, China;

²The Elemental School Affiliated to Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2015-05-18 Revised:2015-08-05 Online:2016-03-01 Published:2016-03-31
Contact: Huiping Mao

摘要/Abstract

摘要： AtPROPEP是拟南芥(Arabidopsis thaliana)具有7个成员的基因家族, 编码内源短肽激素。AtPROPEP基因家族编码的蛋白质C端23个氨基酸短肽能够被2个同源激酶受体AtPEPR1和AtPEPR2识别并结合, 引起下游反应。然而, 对于该家族成员AtPROPEP2,3−6的表达对茉莉酸(JA)和水杨酸(SA)的响应以及在根生长中的作用并不清楚。GUS染色和定量RT-PCR分析结果表明, AtPROPEP2–6的表达对于JA和SA的响应不同, 暗示着它们可能通过不同的方式参与植物的先天免疫反应。AtPROPEP3和AtPROPEP4过表达植株的表型分析表明, AtPROPEP3和AtPROPEP4促进拟南芥根的生长。

Abstract: AtPROPEP is a 7-member gene family in the Arabidopsis genome that encodes an endogenous peptide hormone. The C-terminal of the encoded proteins, about 23 amino acids, can be recognized by the corresponding receptors AtPEPR1 and AtPEPR2, which in turn evoke downstream cascades. Whether AtPROPEP2–6 respond to jasmonic acid and salicylic acid is unknown, as is their roles in root development. In this study, GUS staining and quantitative RT-PCR revealed that AtPROPEP2–6 expression had various responses to jasmonic acid and salicylic acid treatments, which implies their roles in innate immunity. In addition, Arabidopsis overexpressing AtPROPEP3 and At- PROPEP4 had significant longer roots than did the wild type.

李冬梅, 王路雅, 张澜玥,帖子阳, 毛惠平. 拟南芥短肽激素PROPEP基因家族在根生长中的作用机理. 植物学报, 2016, 51(2): 202-209.

Dongmei Li, Luya Wang, Lanyue Zhang, Ziyang Tie, Huiping Mao. Mechanism of Arabidopsis Short Peptide Hormones PROPEP Gene Family in the Root Growth. Chinese Bulletin of Botany, 2016, 51(2): 202-209.

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拟南芥短肽激素PROPEP基因家族在根生长中的作用机理

Mechanism of Arabidopsis Short Peptide Hormones PROPEP Gene Family in the Root Growth

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